↓ Skip to main content

Macrophage Infiltration Is a Causative Factor for Ligamentum Flavum Hypertrophy through the Activation of Collagen Production in Fibroblasts

Overview of attention for article published in American Journal of Pathology, December 2017
Altmetric Badge

About this Attention Score

  • Good Attention Score compared to outputs of the same age (65th percentile)
  • High Attention Score compared to outputs of the same age and source (80th percentile)

Mentioned by

twitter
4 tweeters
facebook
3 Facebook pages

Readers on

mendeley
17 Mendeley
You are seeing a free-to-access but limited selection of the activity Altmetric has collected about this research output. Click here to find out more.
Title
Macrophage Infiltration Is a Causative Factor for Ligamentum Flavum Hypertrophy through the Activation of Collagen Production in Fibroblasts
Published in
American Journal of Pathology, December 2017
DOI 10.1016/j.ajpath.2017.08.020
Pubmed ID
Authors

Takeyuki Saito, Masamitsu Hara, Hiromi Kumamaru, Kazu Kobayakawa, Kazuya Yokota, Ken Kijima, Shingo Yoshizaki, Katsumi Harimaya, Yoshihiro Matsumoto, Kenichi Kawaguchi, Mitsumasa Hayashida, Yutaka Inagaki, Keiichiro Shiba, Yasuharu Nakashima, Seiji Okada

Abstract

Ligamentum flavum (LF) hypertrophy causes lumbar spinal canal stenosis, leading to leg pain and disability in activities of daily living in elderly individuals. Although there have been previous studies on LF hypertrophy, its pathomechanisms have not been fully elucidated. In this study, we demonstrated that infiltrating macrophages were a causative factor for LF hypertrophy. Induction of macrophages into the mouse LF by applying a micro-injury resulted in LF hypertrophy along with collagen accumulation and fibroblasts proliferation at the injured site, which were very similar to the characteristics observed in the severely hypertrophied LF of human. However, we found that macrophage depletion by injecting clodronate-containing liposomes counteracted LF hypertrophy even with micro-injury. For identification of fibroblasts in the LF, we utilized collagen type 1 alpha 2 linked to green fluorescent protein (COL1a2-GFP) transgenic mice, and selectively isolated GFP-positive fibroblasts from the micro-injured LF using laser microdissection (LMD). A quantitative RT-PCR on LMD samples showed that the gene expression of collagen markedly increased in the fibroblasts at the injured site with infiltrating macrophages compared to the uninjured location. These results suggested that macrophage infiltration was crucial for LF hypertrophy by stimulating collagen production in fibroblasts, providing better understanding the pathophysiology of LF hypertrophy.

Twitter Demographics

The data shown below were collected from the profiles of 4 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 17 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 17 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 3 18%
Researcher 3 18%
Student > Doctoral Student 2 12%
Other 2 12%
Professor > Associate Professor 2 12%
Other 5 29%
Readers by discipline Count As %
Medicine and Dentistry 7 41%
Neuroscience 4 24%
Pharmacology, Toxicology and Pharmaceutical Science 2 12%
Biochemistry, Genetics and Molecular Biology 2 12%
Arts and Humanities 1 6%
Other 1 6%

Attention Score in Context

This research output has an Altmetric Attention Score of 4. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 11 December 2017.
All research outputs
#3,592,905
of 12,488,583 outputs
Outputs from American Journal of Pathology
#1,232
of 4,143 outputs
Outputs of similar age
#128,850
of 378,875 outputs
Outputs of similar age from American Journal of Pathology
#10
of 52 outputs
Altmetric has tracked 12,488,583 research outputs across all sources so far. This one has received more attention than most of these and is in the 71st percentile.
So far Altmetric has tracked 4,143 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 5.8. This one has gotten more attention than average, scoring higher than 70% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 378,875 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 65% of its contemporaries.
We're also able to compare this research output to 52 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 80% of its contemporaries.