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Chromatin Immunoprecipitation

Overview of attention for book
Cover of 'Chromatin Immunoprecipitation'

Table of Contents

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    Book Overview
  2. Altmetric Badge
    Chapter 1 ChIP and ChIP-Related Techniques: Expanding the Fields of Application and Improving ChIP Performance
  3. Altmetric Badge
    Chapter 2 Considerations on Experimental Design and Data Analysis of Chromatin Immunoprecipitation Experiments
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    Chapter 3 How to Combine ChIP with qPCR
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    Chapter 4 Analysis of Protein–DNA Interaction by Chromatin Immunoprecipitation and DNA Tiling Microarray (ChIP-on-chip)
  6. Altmetric Badge
    Chapter 5 Chromatin Immunoprecipitation from Mouse Embryonic Tissue or Adherent Cells in Culture, Followed by Next-Generation Sequencing
  7. Altmetric Badge
    Chapter 6 Chromatin RNA Immunoprecipitation (ChRIP)
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    Chapter 7 DNA Accessibility by MNase Digestions
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    Chapter 8 Characterization of the Nucleosome Landscape by Micrococcal Nuclease-Sequencing (MNase-seq)
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    Chapter 9 ChIP-re-ChIP: Co-occupancy Analysis by Sequential Chromatin Immunoprecipitation
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    Chapter 10 Sm-ChIPi: Single-Molecule Chromatin Immunoprecipitation Imaging
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    Chapter 11 Chromatin Immunoprecipitation of Skeletal Muscle Tissue
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    Chapter 12 Chromatin Immunoprecipitation Assay in the Hyperthermoacidophilic Crenarchaeon, Sulfolobus acidocaldarius
  14. Altmetric Badge
    Chapter 13 Using Intra-ChIP to Measure Protein–DNA Interactions in Intracellular Pathogens
  15. Altmetric Badge
    Chapter 14 Native Chromatin Immunoprecipitation-Sequencing (ChIP-Seq) from Low Cell Numbers
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    Chapter 15 MOBE-ChIP: Probing Cell Type-Specific Binding Through Large-Scale Chromatin Immunoprecipitation
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    Chapter 16 Multiplexed ChIP-Seq Using Direct Nucleosome Barcoding: A Tool for High-Throughput Chromatin Analysis
  18. Altmetric Badge
    Chapter 17 Analysis of ChIP-seq Data in R/Bioconductor
  19. Altmetric Badge
    Chapter 18 Spike-In Normalization of ChIP Data Using DNA–DIG–Antibody Complex
Attention for Chapter 16: Multiplexed ChIP-Seq Using Direct Nucleosome Barcoding: A Tool for High-Throughput Chromatin Analysis
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Chapter title
Multiplexed ChIP-Seq Using Direct Nucleosome Barcoding: A Tool for High-Throughput Chromatin Analysis
Chapter number 16
Book title
Chromatin Immunoprecipitation
Published in
Methods in molecular biology, January 2018
DOI 10.1007/978-1-4939-7380-4_16
Pubmed ID
Book ISBNs
978-1-4939-7379-8, 978-1-4939-7380-4
Authors

Christophe D. Chabbert, Sophie H. Adjalley, Lars M. Steinmetz, Vicent Pelechano

Abstract

Chromatin immunoprecipitation followed by sequencing (ChIP-Seq) or microarray hybridization (ChIP-on-chip) are standard methods for the study of transcription factor binding sites and histone chemical modifications. However, these approaches only allow profiling of a single factor or protein modification at a time.In this chapter, we present Bar-ChIP, a higher throughput version of ChIP-Seq that relies on the direct ligation of molecular barcodes to chromatin fragments. Bar-ChIP enables the concurrent profiling of multiple DNA-protein interactions and is therefore amenable to experimental scale-up, without the need for any robotic instrumentation.

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X Demographics

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 14 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 14 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 3 21%
Professor 2 14%
Student > Ph. D. Student 2 14%
Student > Postgraduate 2 14%
Student > Master 2 14%
Other 1 7%
Unknown 2 14%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 9 64%
Agricultural and Biological Sciences 4 29%
Unknown 1 7%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 16 October 2017.
All research outputs
#13,571,666
of 23,005,189 outputs
Outputs from Methods in molecular biology
#3,653
of 13,160 outputs
Outputs of similar age
#219,960
of 442,254 outputs
Outputs of similar age from Methods in molecular biology
#351
of 1,498 outputs
Altmetric has tracked 23,005,189 research outputs across all sources so far. This one is in the 39th percentile – i.e., 39% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,160 research outputs from this source. They receive a mean Attention Score of 3.4. This one has gotten more attention than average, scoring higher than 70% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 442,254 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 48th percentile – i.e., 48% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 1,498 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 74% of its contemporaries.