| Chapter title |
Chromatin RNA Immunoprecipitation (ChRIP)
|
|---|---|
| Chapter number | 6 |
| Book title |
Chromatin Immunoprecipitation
|
| Published in |
Methods in molecular biology, January 2018
|
| DOI | 10.1007/978-1-4939-7380-4_6 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-7379-8, 978-1-4939-7380-4
|
| Authors |
Tanmoy Mondal, Santhilal Subhash, Chandrasekhar Kanduri |
| Abstract |
Researchers have recently had a growing interest in understanding the functional role of long noncoding RNAs (lncRNAs) in chromatin organization. Accumulated evidence suggests lncRNAs could act as interphase molecules between chromatin and chromatin remodelers to define the epigenetic code. However, it is not clear how lncRNAs target chromatin remodelers to specific chromosomal regions in order to establish a functionally distinct epigenetic state of chromatin. We developed and optimized chromatin RNA immunoprecipitation (ChRIP) technology to characterize the lncRNAs associated with active and inactive chromatin compartments. Use of ChRIP to identify chromatin-bound lncRNA will further improve our knowledge regarding the functional role of lncRNAs in establishing epigenetic modifications of chromatin. |
X Demographics
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|---|---|---|
| United States | 4 | 67% |
| Unknown | 2 | 33% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Scientists | 3 | 50% |
| Members of the public | 3 | 50% |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 12 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 4 | 33% |
| Student > Doctoral Student | 1 | 8% |
| Professor | 1 | 8% |
| Student > Bachelor | 1 | 8% |
| Student > Master | 1 | 8% |
| Other | 1 | 8% |
| Unknown | 3 | 25% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 6 | 50% |
| Agricultural and Biological Sciences | 2 | 17% |
| Computer Science | 1 | 8% |
| Social Sciences | 1 | 8% |
| Unknown | 2 | 17% |