| Chapter title |
ChIP-re-ChIP: Co-occupancy Analysis by Sequential Chromatin Immunoprecipitation
|
|---|---|
| Chapter number | 9 |
| Book title |
Chromatin Immunoprecipitation
|
| Published in |
Methods in molecular biology, January 2018
|
| DOI | 10.1007/978-1-4939-7380-4_9 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-7379-8, 978-1-4939-7380-4
|
| Authors |
Timothy V. Beischlag, Gratien G. Prefontaine, Oliver Hankinson |
| Abstract |
Chromatin immunoprecipitation (ChIP) exploits the specific interactions between DNA and DNA-associated proteins. It can be used to examine a wide range of experimental parameters. A number of proteins bound at the same genomic location can identify a multi-protein chromatin complex where several proteins work together to regulate gene transcription or chromatin configuration. In many instances, this can be achieved using sequential ChIP; or simply, ChIP-re-ChIP. Whether it is for the examination of specific transcriptional or epigenetic regulators, or for the identification of cistromes, the ability to perform a sequential ChIP adds a higher level of power and definition to these analyses. In this chapter, we describe a simple and reliable method for the sequential ChIP assay. |
X Demographics
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|---|---|---|
| United States | 1 | 33% |
| Canada | 1 | 33% |
| Unknown | 1 | 33% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 2 | 67% |
| Scientists | 1 | 33% |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 44 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 9 | 20% |
| Researcher | 7 | 16% |
| Student > Master | 6 | 14% |
| Student > Bachelor | 3 | 7% |
| Student > Postgraduate | 3 | 7% |
| Other | 8 | 18% |
| Unknown | 8 | 18% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 19 | 43% |
| Agricultural and Biological Sciences | 7 | 16% |
| Psychology | 2 | 5% |
| Immunology and Microbiology | 2 | 5% |
| Pharmacology, Toxicology and Pharmaceutical Science | 1 | 2% |
| Other | 5 | 11% |
| Unknown | 8 | 18% |