| Chapter title |
Chromatin Immunoprecipitation from Mouse Embryonic Tissue or Adherent Cells in Culture, Followed by Next-Generation Sequencing
|
|---|---|
| Chapter number | 5 |
| Book title |
Chromatin Immunoprecipitation
|
| Published in |
Methods in molecular biology, January 2018
|
| DOI | 10.1007/978-1-4939-7380-4_5 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-7379-8, 978-1-4939-7380-4
|
| Authors |
Mário A. F. Soares, Diogo S. Castro |
| Abstract |
Chromatin immunoprecipitation (ChIP) is considered the method of choice for characterizing interactions between a protein of interest and specific genomic regions. It is of paramount importance in gene-regulation studies, as it can be used to map the target regions of sequence-specific transcription factors and cofactors, or histone marks that characterize distinct chromatin states. ChIP can be used directly to probe interactions with candidate regions (ChIP-PCR), or coupled to Next-Generation Sequencing (ChIP-seq) to generate genome-wide information. This chapter describes a protocol for performing ChIP and ChIP-seq of transcription factors, starting either from mouse embryonic tissue or adherent cells in culture. |
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Demographic breakdown
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|---|---|---|
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