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Chromatin Immunoprecipitation

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Cover of 'Chromatin Immunoprecipitation'

Table of Contents

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    Book Overview
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    Chapter 1 ChIP and ChIP-Related Techniques: Expanding the Fields of Application and Improving ChIP Performance
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    Chapter 2 Considerations on Experimental Design and Data Analysis of Chromatin Immunoprecipitation Experiments
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    Chapter 3 How to Combine ChIP with qPCR
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    Chapter 4 Analysis of Protein–DNA Interaction by Chromatin Immunoprecipitation and DNA Tiling Microarray (ChIP-on-chip)
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    Chapter 5 Chromatin Immunoprecipitation from Mouse Embryonic Tissue or Adherent Cells in Culture, Followed by Next-Generation Sequencing
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    Chapter 6 Chromatin RNA Immunoprecipitation (ChRIP)
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    Chapter 7 DNA Accessibility by MNase Digestions
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    Chapter 8 Characterization of the Nucleosome Landscape by Micrococcal Nuclease-Sequencing (MNase-seq)
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    Chapter 9 ChIP-re-ChIP: Co-occupancy Analysis by Sequential Chromatin Immunoprecipitation
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    Chapter 10 Sm-ChIPi: Single-Molecule Chromatin Immunoprecipitation Imaging
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    Chapter 11 Chromatin Immunoprecipitation of Skeletal Muscle Tissue
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    Chapter 12 Chromatin Immunoprecipitation Assay in the Hyperthermoacidophilic Crenarchaeon, Sulfolobus acidocaldarius
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    Chapter 13 Using Intra-ChIP to Measure Protein–DNA Interactions in Intracellular Pathogens
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    Chapter 14 Native Chromatin Immunoprecipitation-Sequencing (ChIP-Seq) from Low Cell Numbers
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    Chapter 15 MOBE-ChIP: Probing Cell Type-Specific Binding Through Large-Scale Chromatin Immunoprecipitation
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    Chapter 16 Multiplexed ChIP-Seq Using Direct Nucleosome Barcoding: A Tool for High-Throughput Chromatin Analysis
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    Chapter 17 Analysis of ChIP-seq Data in R/Bioconductor
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    Chapter 18 Spike-In Normalization of ChIP Data Using DNA–DIG–Antibody Complex
Attention for Chapter 13: Using Intra-ChIP to Measure Protein–DNA Interactions in Intracellular Pathogens
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Chapter title
Using Intra-ChIP to Measure Protein–DNA Interactions in Intracellular Pathogens
Chapter number 13
Book title
Chromatin Immunoprecipitation
Published in
Methods in molecular biology, January 2018
DOI 10.1007/978-1-4939-7380-4_13
Pubmed ID
Book ISBNs
978-1-4939-7379-8, 978-1-4939-7380-4
Authors

Brett R. Hanson, Ming Tan

Abstract

Chromatin immunoprecipitation is used to measure the binding of transcription factors to target DNA sequences in order to better understand transcriptional regulation. Here, we describe a process to analyze bacterial transcription factor binding in the context of an infected eukaryotic host cell. Using this approach, we measured the binding kinetics of three Chlamydia trachomatis transcription factors within infected cells, and demonstrated temporal changes in binding.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 6 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 6 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 2 33%
Student > Doctoral Student 2 33%
Student > Postgraduate 1 17%
Student > Master 1 17%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 3 50%
Nursing and Health Professions 1 17%
Medicine and Dentistry 1 17%
Unknown 1 17%