Title |
An SNP-based saturated genetic map and QTL analysis of fruit-related traits in cucumber using specific-length amplified fragment (SLAF) sequencing
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Published in |
BMC Genomics, December 2014
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DOI | 10.1186/1471-2164-15-1158 |
Pubmed ID | |
Authors |
Qingzhen Wei, Yunzhu Wang, Xiaodong Qin, Yunxia Zhang, Zhentao Zhang, Jing Wang, Ji Li, Qunfeng Lou, Jinfeng Chen |
Abstract |
Cucumber, Cucumis sativus L., is an economically important vegetable crop which is processed or consumed fresh worldwide. However, the narrow genetic base in cucumber makes it difficult for constructing high-density genetic maps. The development of massively parallel genotyping methods and next-generation sequencing (NGS) technologies provides an excellent opportunity for developing single nucleotide polymorphisms (SNPs) for linkage map construction and QTL analysis of horticultural traits. Specific-length amplified fragment sequencing (SLAF-seq) is a recent marker development technology that allows large-scale SNP discovery and genotyping at a reasonable cost. In this study, we constructed a high-density SNP map for cucumber using SLAF-seq and detected fruit-related QTLs. |
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