Title |
The dynamic dimer structure of the chaperone Trigger Factor
|
---|---|
Published in |
Nature Communications, December 2017
|
DOI | 10.1038/s41467-017-02196-7 |
Pubmed ID | |
Authors |
Leonor Morgado, Björn M. Burmann, Timothy Sharpe, Adam Mazur, Sebastian Hiller |
Abstract |
The chaperone Trigger Factor (TF) from Escherichia coli forms a dimer at cellular concentrations. While the monomer structure of TF is well known, the spatial arrangement of this dimeric chaperone storage form has remained unclear. Here, we determine its structure by a combination of high-resolution NMR spectroscopy and biophysical methods. TF forms a symmetric head-to-tail dimer, where the ribosome binding domain is in contact with the substrate binding domain, while the peptidyl-prolyl isomerase domain contributes only slightly to the dimer affinity. The dimer structure is highly dynamic, with the two ribosome binding domains populating a conformational ensemble in the center. These dynamics result from intermolecular in trans interactions of the TF client-binding site with the ribosome binding domain, which is conformationally frustrated in the absence of the ribosome. The avidity in the dimer structure explains how the dimeric state of TF can be monomerized also by weakly interacting clients. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 1 | 50% |
Unknown | 1 | 50% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 2 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 54 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 15 | 28% |
Researcher | 9 | 17% |
Student > Bachelor | 5 | 9% |
Student > Postgraduate | 3 | 6% |
Student > Master | 3 | 6% |
Other | 6 | 11% |
Unknown | 13 | 24% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 19 | 35% |
Agricultural and Biological Sciences | 7 | 13% |
Chemistry | 6 | 11% |
Earth and Planetary Sciences | 2 | 4% |
Nursing and Health Professions | 1 | 2% |
Other | 4 | 7% |
Unknown | 15 | 28% |