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Expression of adhA from different organisms in Clostridium thermocellum

Overview of attention for article published in Biotechnology for Biofuels and Bioproducts, November 2017
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Title
Expression of adhA from different organisms in Clostridium thermocellum
Published in
Biotechnology for Biofuels and Bioproducts, November 2017
DOI 10.1186/s13068-017-0940-8
Pubmed ID
Authors

Tianyong Zheng, Jingxuan Cui, Hye Ri Bae, Lee R. Lynd, Daniel G. Olson

Abstract

Clostridium thermocellum is a cellulolytic anaerobic thermophile that is a promising candidate for consolidated bioprocessing of lignocellulosic biomass into biofuels such as ethanol. It was previously shown that expressing Thermoanaerobacterium saccharolyticum adhA in C. thermocellum increases ethanol yield.In this study, we investigated expression of adhA genes from different organisms in Clostridium thermocellum. Based on sequence identity to T. saccharolyticum adhA, we chose adhA genes from 10 other organisms: Clostridium botulinum, Methanocaldococcus bathoardescens, Thermoanaerobacterium ethanolicus, Thermoanaerobacter mathranii, Thermococcus strain AN1, Thermoanaerobacterium thermosaccharolyticum, Caldicellulosiruptor saccharolyticus, Fervidobacterium nodosum, Marinitoga piezophila, and Thermotoga petrophila. All 11 adhA genes (including T. saccharolyticum adhA) were expressed in C. thermocellum and fermentation end products were analyzed. All 11 adhA genes increased C. thermocellum ethanol yield compared to the empty-vector control. C. botulinum and T. ethanolicus adhA genes generated significantly higher ethanol yield than T. saccharolyticum adhA. Our results indicated that expressing adhA is an effective method of increasing ethanol yield in wild-type C. thermocellum, and that this appears to be a general property of adhA genes.

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Mendeley readers

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Geographical breakdown

Country Count As %
Unknown 24 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 4 17%
Researcher 3 13%
Student > Doctoral Student 2 8%
Professor > Associate Professor 2 8%
Student > Master 2 8%
Other 5 21%
Unknown 6 25%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 6 25%
Agricultural and Biological Sciences 6 25%
Environmental Science 1 4%
Nursing and Health Professions 1 4%
Immunology and Microbiology 1 4%
Other 3 13%
Unknown 6 25%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 08 December 2017.
All research outputs
#20,663,600
of 25,382,440 outputs
Outputs from Biotechnology for Biofuels and Bioproducts
#1,285
of 1,578 outputs
Outputs of similar age
#339,298
of 445,786 outputs
Outputs of similar age from Biotechnology for Biofuels and Bioproducts
#28
of 41 outputs
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So far Altmetric has tracked 1,578 research outputs from this source. They receive a mean Attention Score of 4.9. This one is in the 10th percentile – i.e., 10% of its peers scored the same or lower than it.
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We're also able to compare this research output to 41 others from the same source and published within six weeks on either side of this one. This one is in the 24th percentile – i.e., 24% of its contemporaries scored the same or lower than it.