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Application of Fragment-Based Screening to the Design of Inhibitors of Escherichia coli DsbA

Overview of attention for article published in Angewandte Chemie. International Edition, December 2014
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About this Attention Score

  • In the top 5% of all research outputs scored by Altmetric
  • High Attention Score compared to outputs of the same age (95th percentile)
  • High Attention Score compared to outputs of the same age and source (95th percentile)

Mentioned by

blogs
3 blogs
twitter
4 tweeters
facebook
2 Facebook pages
wikipedia
1 Wikipedia page

Citations

dimensions_citation
23 Dimensions

Readers on

mendeley
60 Mendeley
citeulike
1 CiteULike
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Title
Application of Fragment-Based Screening to the Design of Inhibitors of Escherichia coli DsbA
Published in
Angewandte Chemie. International Edition, December 2014
DOI 10.1002/anie.201410341
Pubmed ID
Authors

Luke A. Adams, Pooja Sharma, Biswaranjan Mohanty, Olga V. Ilyichova, Mark D. Mulcair, Martin L. Williams, Ellen C. Gleeson, Makrina Totsika, Bradley C. Doak, Sofia Caria, Kieran Rimmer, James Horne, Stephen R. Shouldice, Mansha Vazirani, Stephen J. Headey, Brent R. Plumb, Jennifer L. Martin, Begoña Heras, Jamie S. Simpson, Martin J. Scanlon

Abstract

The thiol-disulfide oxidoreductase enzyme DsbA catalyzes the formation of disulfide bonds in the periplasm of Gram-negative bacteria. DsbA substrates include proteins involved in bacterial virulence. In the absence of DsbA, many of these proteins do not fold correctly, which renders the bacteria avirulent. Thus DsbA is a critical mediator of virulence and inhibitors may act as antivirulence agents. Biophysical screening has been employed to identify fragments that bind to DsbA from Escherichia coli. Elaboration of one of these fragments produced compounds that inhibit DsbA activity in vitro. In cell-based assays, the compounds inhibit bacterial motility, but have no effect on growth in liquid culture, which is consistent with selective inhibition of DsbA. Crystal structures of inhibitors bound to DsbA indicate that they bind adjacent to the active site. Together, the data suggest that DsbA may be amenable to the development of novel antibacterial compounds that act by inhibiting bacterial virulence.

Twitter Demographics

The data shown below were collected from the profiles of 4 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 60 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Spain 1 2%
Germany 1 2%
Australia 1 2%
Unknown 57 95%

Demographic breakdown

Readers by professional status Count As %
Researcher 17 28%
Student > Ph. D. Student 9 15%
Student > Postgraduate 8 13%
Student > Master 6 10%
Student > Bachelor 6 10%
Other 11 18%
Unknown 3 5%
Readers by discipline Count As %
Chemistry 25 42%
Agricultural and Biological Sciences 11 18%
Biochemistry, Genetics and Molecular Biology 9 15%
Pharmacology, Toxicology and Pharmaceutical Science 6 10%
Immunology and Microbiology 2 3%
Other 3 5%
Unknown 4 7%

Attention Score in Context

This research output has an Altmetric Attention Score of 32. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 23 October 2016.
All research outputs
#431,188
of 12,180,133 outputs
Outputs from Angewandte Chemie. International Edition
#866
of 29,293 outputs
Outputs of similar age
#12,069
of 274,860 outputs
Outputs of similar age from Angewandte Chemie. International Edition
#28
of 674 outputs
Altmetric has tracked 12,180,133 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 96th percentile: it's in the top 5% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 29,293 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 6.8. This one has done particularly well, scoring higher than 97% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 274,860 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 95% of its contemporaries.
We're also able to compare this research output to 674 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 95% of its contemporaries.