Chapter title |
Multiplexing for Oxidative Bisulfite Sequencing (oxBS-seq)
|
---|---|
Chapter number | 34 |
Book title |
DNA Methylation Protocols
|
Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-7481-8_34 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7479-5, 978-1-4939-7481-8
|
Authors |
Kristina Kirschner, Felix Krueger, Anthony R. Green, Tamir Chandra |
Abstract |
DNA modifications, especially methylation, are known to play a crucial part in many regulatory processes in the cell. Recently, 5-hydroxymethylcytosine (5hmC) was discovered, a DNA modification derived as an intermediate of 5-methylcytosine (5mC) oxidation. Efforts to gain insights into function of this DNA modification are underway and several methods were recently described to assess 5hmC levels using sequencing approaches. Here we integrate adaptation based multiplexing and high-efficiency library prep into the oxidative Bisulfite Sequencing (oxBS-seq) workflow reducing the starting amount and cost per sample to identify 5hmC levels genome-wide. |
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