| Chapter title |
Sampling, Extraction, and High-Throughput Sequencing Methods for Environmental Microbial and Viral Communities
|
|---|---|
| Chapter number | 11 |
| Book title |
Next Generation Sequencing
|
| Published in |
Methods in molecular biology, January 2018
|
| DOI | 10.1007/978-1-4939-7514-3_11 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-7512-9, 978-1-4939-7514-3
|
| Authors |
Pedro J. Torres, Scott T. Kelley |
| Abstract |
The emergence of high-throughput sequencing technologies has deepened our understanding of complex microbial communities and greatly facilitated the study of as-yet uncultured microbes and viruses. Studies of complex microbial communities require high-quality data to generate valid results. Here, we detail current methods of microbial and viral community sample acquisition, DNA extraction, sample preparation, and sequencing on Illumina high-throughput platforms. While using appropriate analytical tools is important, it must not overshadow the need for establishing a proper experimental design and obtaining sufficient numbers of samples for statistical purposes. Researchers must also take care to sample biologically relevant sites and control for potential confounding factors (e.g., contamination). |
X Demographics
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 4 | 57% |
| Unknown | 3 | 43% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 5 | 71% |
| Scientists | 2 | 29% |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 12 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Postgraduate | 3 | 25% |
| Professor | 2 | 17% |
| Student > Ph. D. Student | 2 | 17% |
| Other | 1 | 8% |
| Student > Doctoral Student | 1 | 8% |
| Other | 2 | 17% |
| Unknown | 1 | 8% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 4 | 33% |
| Agricultural and Biological Sciences | 3 | 25% |
| Nursing and Health Professions | 1 | 8% |
| Environmental Science | 1 | 8% |
| Social Sciences | 1 | 8% |
| Other | 1 | 8% |
| Unknown | 1 | 8% |