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Next Generation Sequencing

Overview of attention for book
Cover of 'Next Generation Sequencing'

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter 1 An Integrated Polysome Profiling and Ribosome Profiling Method to Investigate In Vivo Translatome
  3. Altmetric Badge
    Chapter 2 Measuring Nascent Transcripts by Nascent-seq
  4. Altmetric Badge
    Chapter 3 Genome-Wide Copy Number Alteration Detection in Preimplantation Genetic Diagnosis
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    Chapter 4 Multiplexed Targeted Sequencing for Oxford Nanopore MinION: A Detailed Library Preparation Procedure
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    Chapter 5 Hi-Plex for Simple, Accurate, and Cost-Effective Amplicon-based Targeted DNA Sequencing
  7. Altmetric Badge
    Chapter 6 ClickSeq: Replacing Fragmentation and Enzymatic Ligation with Click-Chemistry to Prevent Sequence Chimeras
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    Chapter 7 Genome-Wide Analysis of DNA Methylation in Single Cells Using a Post-bisulfite Adapter Tagging Approach
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    Chapter 8 Sequencing of Genomes from Environmental Single Cells
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    Chapter 9 SNP Discovery from Single and Multiplex Genome Assemblies of Non-model Organisms
  11. Altmetric Badge
    Chapter 10 CleanTag Adapters Improve Small RNA Next-Generation Sequencing Library Preparation by Reducing Adapter Dimers
  12. Altmetric Badge
    Chapter 11 Sampling, Extraction, and High-Throughput Sequencing Methods for Environmental Microbial and Viral Communities
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    Chapter 12 A Bloody Primer: Analysis of RNA-Seq from Tissue Admixtures
  14. Altmetric Badge
    Chapter 13 Next-Generation Sequencing of Genome-Wide CRISPR Screens
  15. Altmetric Badge
    Chapter 14 Gene Profiling and T Cell Receptor Sequencing from Antigen-Specific CD4 T Cells
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    Chapter 15 Investigate Global Chromosomal Interaction by Hi-C in Human Naive CD4 T Cells
  17. Altmetric Badge
    Chapter 16 Primer Extension, Capture, and On-Bead cDNA Ligation: An Efficient RNAseq Library Prep Method for Determining Reverse Transcription Termination Sites
Attention for Chapter 3: Genome-Wide Copy Number Alteration Detection in Preimplantation Genetic Diagnosis
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Chapter title
Genome-Wide Copy Number Alteration Detection in Preimplantation Genetic Diagnosis
Chapter number 3
Book title
Next Generation Sequencing
Published in
Methods in molecular biology, January 2018
DOI 10.1007/978-1-4939-7514-3_3
Pubmed ID
Book ISBNs
978-1-4939-7512-9, 978-1-4939-7514-3
Authors

Lieselot Deleye, Dieter De Coninck, Dieter Deforce, Filip Van Nieuwerburgh

Abstract

Shallow whole genome sequencing has recently been introduced for genome-wide detection of chromosomal copy number alterations (CNAs) in preimplantation genetic diagnosis (PGD), using only 4-7 trophectoderm cells biopsied from day-5 embryos. This chapter describes the complete method, starting from whole genome amplification (WGA) on isolated blastomere(s), up to data analysis for CNA detection. The process is described generically and can also be used to perform CNA analysis on a limited number of cells (down to a single cell) in other applications. This unique description also includes some tips and tricks to increase the chance of success.

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X Demographics

The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 4 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 4 100%

Demographic breakdown

Readers by professional status Count As %
Unspecified 1 25%
Student > Doctoral Student 1 25%
Unknown 2 50%
Readers by discipline Count As %
Unspecified 1 25%
Agricultural and Biological Sciences 1 25%
Unknown 2 50%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 11 December 2017.
All research outputs
#18,578,649
of 23,011,300 outputs
Outputs from Methods in molecular biology
#7,962
of 13,157 outputs
Outputs of similar age
#330,510
of 442,319 outputs
Outputs of similar age from Methods in molecular biology
#950
of 1,498 outputs
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