Chapter title |
Genome-Wide Copy Number Alteration Detection in Preimplantation Genetic Diagnosis
|
---|---|
Chapter number | 3 |
Book title |
Next Generation Sequencing
|
Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-7514-3_3 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7512-9, 978-1-4939-7514-3
|
Authors |
Lieselot Deleye, Dieter De Coninck, Dieter Deforce, Filip Van Nieuwerburgh |
Abstract |
Shallow whole genome sequencing has recently been introduced for genome-wide detection of chromosomal copy number alterations (CNAs) in preimplantation genetic diagnosis (PGD), using only 4-7 trophectoderm cells biopsied from day-5 embryos. This chapter describes the complete method, starting from whole genome amplification (WGA) on isolated blastomere(s), up to data analysis for CNA detection. The process is described generically and can also be used to perform CNA analysis on a limited number of cells (down to a single cell) in other applications. This unique description also includes some tips and tricks to increase the chance of success. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
Austria | 1 | 50% |
Unknown | 1 | 50% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 2 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 4 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Unspecified | 1 | 25% |
Student > Doctoral Student | 1 | 25% |
Unknown | 2 | 50% |
Readers by discipline | Count | As % |
---|---|---|
Unspecified | 1 | 25% |
Agricultural and Biological Sciences | 1 | 25% |
Unknown | 2 | 50% |