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AM404, paracetamol metabolite, prevents prostaglandin synthesis in activated microglia by inhibiting COX activity

Overview of attention for article published in Journal of Neuroinflammation, December 2017
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About this Attention Score

  • Above-average Attention Score compared to outputs of the same age (58th percentile)
  • Good Attention Score compared to outputs of the same age and source (66th percentile)

Mentioned by

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5 tweeters

Citations

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11 Dimensions

Readers on

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30 Mendeley
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Title
AM404, paracetamol metabolite, prevents prostaglandin synthesis in activated microglia by inhibiting COX activity
Published in
Journal of Neuroinflammation, December 2017
DOI 10.1186/s12974-017-1014-3
Pubmed ID
Authors

Soraya Wilke Saliba, Ariel R. Marcotegui, Ellen Fortwängler, Johannes Ditrich, Juan Carlos Perazzo, Eduardo Muñoz, Antônio Carlos Pinheiro de Oliveira, Bernd L. Fiebich

Abstract

N-arachidonoylphenolamine (AM404), a paracetamol metabolite, is a potent agonist of the transient receptor potential vanilloid type 1 (TRPV1) and low-affinity ligand of the cannabinoid receptor type 1 (CB1). There is evidence that AM404 exerts its pharmacological effects in immune cells. However, the effect of AM404 on the production of inflammatory mediators of the arachidonic acid pathway in activated microglia is still not fully elucidated. In the present study, we investigated the effects of AM404 on the eicosanoid production induced by lipopolysaccharide (LPS) in organotypic hippocampal slices culture (OHSC) and primary microglia cultures using Western blot, immunohistochemistry, and ELISA. Our results show that AM404 inhibited LPS-mediated prostaglandin E2 (PGE2) production in OHSC, and LPS-stimulated PGE2 release was totally abolished in OHSC if microglial cells were removed. In primary microglia cultures, AM404 led to a significant dose-dependent decrease in the release of PGE2, independent of TRPV1 or CB1 receptors. Moreover, AM404 also inhibited the production of PGD2 and the formation of reactive oxygen species (8-iso-PGF2 alpha) with a reversible reduction of COX-1- and COX-2 activity. Also, it slightly decreased the levels of LPS-induced COX-2 protein, although no effect was observed on LPS-induced mPGES-1 protein synthesis. This study provides new significant insights about the potential anti-inflammatory role of AM404 and new mechanisms of action of paracetamol on the modulation of prostaglandin production by activated microglia.

Twitter Demographics

The data shown below were collected from the profiles of 5 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 30 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 30 100%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 7 23%
Student > Ph. D. Student 6 20%
Unspecified 6 20%
Student > Master 4 13%
Researcher 3 10%
Other 4 13%
Readers by discipline Count As %
Unspecified 8 27%
Pharmacology, Toxicology and Pharmaceutical Science 7 23%
Biochemistry, Genetics and Molecular Biology 4 13%
Neuroscience 3 10%
Veterinary Science and Veterinary Medicine 2 7%
Other 6 20%

Attention Score in Context

This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 19 December 2017.
All research outputs
#6,608,827
of 12,437,358 outputs
Outputs from Journal of Neuroinflammation
#566
of 1,429 outputs
Outputs of similar age
#144,801
of 360,956 outputs
Outputs of similar age from Journal of Neuroinflammation
#43
of 132 outputs
Altmetric has tracked 12,437,358 research outputs across all sources so far. This one is in the 46th percentile – i.e., 46% of other outputs scored the same or lower than it.
So far Altmetric has tracked 1,429 research outputs from this source. They receive a mean Attention Score of 5.0. This one has gotten more attention than average, scoring higher than 59% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 360,956 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 58% of its contemporaries.
We're also able to compare this research output to 132 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 66% of its contemporaries.