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Extraction of amplifiable DNA from embalmed human cadaver tissue

Overview of attention for article published in BMC Research Notes, December 2017
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2 tweeters

Citations

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5 Dimensions

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16 Mendeley
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Title
Extraction of amplifiable DNA from embalmed human cadaver tissue
Published in
BMC Research Notes, December 2017
DOI 10.1186/s13104-017-3066-y
Pubmed ID
Authors

Lindsay Gielda, Stefanie Rigg

Abstract

The expansion of molecular techniques in medical diagnosis, forensics, and education requires the development of improved techniques of DNA extraction from fixed tissues. Cadaver tissues are not commonly used for genetic analysis due to DNA degradation resulting from the embalming fixation. Modification of existing techniques of tissue disruption combined with phenol-chloroform treatment was done to produce an efficient method of extracting amplifiable DNA of high quality and quantity from non-paraffin embedded embalmed cadaver tissue. Tissues (cerebellum, cerebral cortex, heart, and bone) from four cadavers were used to develop a procedure for DNA isolation, which includes a high heat treatment. The location and age of the tissue had a significant effect on the quantity of DNA recovered. Targeted PCR amplification of the Apolipoprotein gene was used to assess the efficacy of genotypic analysis from the recovered DNA. We report the development of a simple, reliable, and low-cost method of DNA isolation utilizing brain tissue from embalmed tissues that could be used for PCR amplification and genetic analysis.

Twitter Demographics

The data shown below were collected from the profiles of 2 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 16 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 16 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 4 25%
Student > Bachelor 3 19%
Student > Ph. D. Student 3 19%
Other 2 13%
Student > Master 1 6%
Other 1 6%
Unknown 2 13%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 10 63%
Medicine and Dentistry 4 25%
Chemistry 1 6%
Unknown 1 6%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 22 December 2017.
All research outputs
#9,460,910
of 12,341,991 outputs
Outputs from BMC Research Notes
#1,765
of 2,742 outputs
Outputs of similar age
#226,732
of 347,067 outputs
Outputs of similar age from BMC Research Notes
#149
of 301 outputs
Altmetric has tracked 12,341,991 research outputs across all sources so far. This one is in the 20th percentile – i.e., 20% of other outputs scored the same or lower than it.
So far Altmetric has tracked 2,742 research outputs from this source. They receive a mean Attention Score of 4.4. This one is in the 30th percentile – i.e., 30% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 347,067 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 28th percentile – i.e., 28% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 301 others from the same source and published within six weeks on either side of this one. This one is in the 40th percentile – i.e., 40% of its contemporaries scored the same or lower than it.