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mRNA Decay

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Cover of 'mRNA Decay'

Table of Contents

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    Book Overview
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    Chapter 1 5′-Bromouridine IP Chase (BRIC)-Seq to Determine RNA Half-Lives
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    Chapter 2 Determining mRNA Decay Rates Using RNA Approach to Equilibrium Sequencing (RATE-Seq)
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    Chapter 3 Metabolic Labeling of Newly Synthesized RNA with 4sU to in Parallel Assess RNA Transcription and Decay
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    Chapter 4 Measuring mRNA Decay in Budding Yeast Using Single Molecule FISH
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    Chapter 5 PAR-CLIP for Discovering Target Sites of RNA-Binding Proteins
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    Chapter 6 Characterizing mRNA Sequence Motifs in the 3′-UTR Using GFP Reporter Constructs
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    Chapter 7 iCLIP of the PIWI Protein Aubergine in Drosophila Embryos
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    Chapter 8 Integration of ENCODE RNAseq and eCLIP Data Sets
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    Chapter 9 Identifying miRNA Targets Using AGO-RIPseq
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    Chapter 10 Integrated Analysis of miRNA and mRNA Expression Profiles to Identify miRNA Targets
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    Chapter 11 Identifying RISC Components Using Ago2 Immunoprecipitation and Mass Spectrometry
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    Chapter 12 Using Tet-Off Cells and RNAi Knockdown to Assay mRNA Decay
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    Chapter 13 Identifying Cellular Nonsense-Mediated mRNA Decay (NMD) Targets: Immunoprecipitation of Phosphorylated UPF1 Followed by RNA Sequencing (p-UPF1 RIP−Seq)
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    Chapter 14 Generation of Cell Lines Stably Expressing a Fluorescent Reporter of Nonsense-Mediated mRNA Decay Activity
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    Chapter 15 Reactivation Assay to Identify Direct Targets of the Nonsense-Mediated mRNA Decay Pathway in Drosophila
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    Chapter 16 Studying Nonsense-Mediated mRNA Decay in Mammalian Cells Using a Multicolored Bioluminescence-Based Reporter System
Attention for Chapter 6: Characterizing mRNA Sequence Motifs in the 3′-UTR Using GFP Reporter Constructs
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Chapter title
Characterizing mRNA Sequence Motifs in the 3′-UTR Using GFP Reporter Constructs
Chapter number 6
Book title
Methods in Molecular Biology
Published in
Methods in molecular biology, December 2017
DOI 10.1007/978-1-4939-7540-2_6
Pubmed ID
Book ISBNs
978-1-4939-7539-6, 978-1-4939-7540-2
Authors

Geissler, Rene, Grimson, Andrew, Rene Geissler, Andrew Grimson

Abstract

GFP reporter constructs are widely used as an expression system for studying the function of regulatory sequence motifs (cis elements) within the 3'-UTRs (3' untranslated regions) of mRNAs. Here we provide details on the characterization of individual sequence motifs, which typically regulate mRNA decay and translation. In addition, we describe methods to identify trans factors required for the function of such elements. To facilitate efficient identification of novel functional 3'-UTR motifs, we describe a screening approach based on dual-color fluorescence reporter constructs. Such screening approaches can be used to test large collections of defined sequence or libraries of random sequences.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 11 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 11 100%

Demographic breakdown

Readers by professional status Count As %
Student > Doctoral Student 3 27%
Researcher 3 27%
Student > Ph. D. Student 1 9%
Student > Bachelor 1 9%
Professor > Associate Professor 1 9%
Other 0 0%
Unknown 2 18%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 5 45%
Agricultural and Biological Sciences 2 18%
Business, Management and Accounting 1 9%
Unknown 3 27%