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Mendeley readers
Chapter title |
Generation of Cell Lines Stably Expressing a Fluorescent Reporter of Nonsense-Mediated mRNA Decay Activity
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Chapter number | 14 |
Book title |
mRNA Decay
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Published in |
Methods in molecular biology, January 2018
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DOI | 10.1007/978-1-4939-7540-2_14 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7539-6, 978-1-4939-7540-2
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Authors |
Nadezhda M. Markina, Anton P. Pereverzev, Dmitry B. Staroverov, Konstantin A. Lukyanov, Nadya G. Gurskaya |
Abstract |
Nonsense-mediated mRNA decay (NMD) is a mechanism of mRNA surveillance ubiquitous among eukaryotes. Importantly, NMD not only removes aberrant transcripts with premature stop codons, but also regulates expression of many normal genes. A recently introduced dual-color fluorescent protein-based reporter enables analysis of NMD activity in live cells. In this chapter we describe the method to generate stable transgenic cell lines expressing the splicing-dependent NMD reporter using consecutive steps of lentivirus transduction and Tol2 transposition. |
Mendeley readers
The data shown below were compiled from readership statistics for 4 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 4 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Professor | 1 | 25% |
Researcher | 1 | 25% |
Unknown | 2 | 50% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 1 | 25% |
Agricultural and Biological Sciences | 1 | 25% |
Unknown | 2 | 50% |