| Chapter title |
Resolving bottlenecks for recombinant protein expression in E. coli.
|
|---|---|
| Chapter number | 12 |
| Book title |
Chemical Genomics and Proteomics
|
| Published in |
Methods in molecular biology, October 2011
|
| DOI | 10.1007/978-1-61779-349-3_12 |
| Pubmed ID | |
| Book ISBNs |
978-1-61779-348-6, 978-1-61779-349-3
|
| Authors |
Peleg Y, Unger T, Yoav Peleg, Tamar Unger |
| Abstract |
Escherichia coli is widely used as an expression system for production of recombinant proteins of prokaryotic and eukaryotic origin. A large body of knowledge has accumulated throughout the last few decades regarding expression of recombinant proteins in E. coli. However, despite this progress, protein production, primarily of eukaryotic origin, still remains a challenge. The biggest obstacle lies in obtaining large amounts of a given protein in a correctly folded form. Several strategies are being used to increase both yield and solubility. These include expression as fusion proteins, co-expression with molecular chaperones, or with a protein partner(s), and the use of multiple constructs for each protein. In this chapter, we focus on strategies for creating expression vectors, as well as on guidelines for improving recombinant protein solubility. |
X Demographics
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Demographic breakdown
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Mendeley readers
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