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Evaluation of caries-associated virulence of biofilms from Candida albicans isolated from saliva of pediatric patients with sickle-cell anemia

Overview of attention for article published in Journal of Applied Oral Science, December 2014
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Title
Evaluation of caries-associated virulence of biofilms from Candida albicans isolated from saliva of pediatric patients with sickle-cell anemia
Published in
Journal of Applied Oral Science, December 2014
DOI 10.1590/1678-775720130603
Pubmed ID
Authors

Fernanda Lourenção BRIGHENTI, Amanda Coelho MEDEIROS, Bruno Mello MATOS, Zulene Eveline Abreu RIBEIRO, Cristiane Yumi KOGA-ITO

Abstract

A previous study demonstrated that the amount of Candida spp. in saliva is higher in children with sickle-cell disease. The results from a recent study demonstrate its participation in the etiology of dental caries. Objective This study assessed caries-associated virulence (production of acid, extracellular polysaccharides, proteins and metabolic activity) of biofilms from Candida albicans isolated from saliva of patients with sickle-cell anemia in comparison to isolates obtained from matched healthy children. Material and Methods The isolates were previously obtained from 25 children (4-6 years) and their matched controls (healthy children). One isolate of C. albicans per children was used, totaling 25 isolates per group. The C. albicans biofilms were grown for five days and analyzed regarding the production of lactic acid, extracellular polysaccharides, proteins and metabolic activity. The production of lactic acid was determined by the enzymatic method. The concentration of extracellular polysaccharides was determined by the phenol-sulphuric acid method, and the concentration of the protein was analyzed using the QuantiPro BCA kit. The XTT reduction was used to verify the metabolic activity. The data were analyzed with GraphPad Prism at 5%. Results The Mean±standard deviation for acid production, extracellular polysaccharides, proteins and metabolic activity of isolates from sickle-cell group was, respectively: 7.1±5.0 mmol/L; 15.6±2.5 μg glucose/mg biofilm; 7,503±3,097 μg/mL; A490 3.5±0.7. For isolates from control group the values obtained were: 3.5±3.3 mmol/L; 12.8±3.4 μg glucose/mg biofilm; 4,995±682 μg/mL; A490 3.4±0.5. The C. albicans isolates from patients with sickle-cell anemia produced a significantly greater quantity of acids (p=0.025), polysaccharides (p=0.025) and proteins (p=0.047) compared with the isolates from control group. However, there was no difference in metabolic activity (XTT) between groups (p=0.750). Conclusion The C. albicans biofilms from patients with sickle-cell anemia presented a greater caries-associated virulence than isolates from healthy children.

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Mendeley readers

The data shown below were compiled from readership statistics for 46 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
India 1 2%
Unknown 45 98%

Demographic breakdown

Readers by professional status Count As %
Researcher 7 15%
Student > Bachelor 6 13%
Student > Postgraduate 5 11%
Student > Master 5 11%
Student > Doctoral Student 2 4%
Other 7 15%
Unknown 14 30%
Readers by discipline Count As %
Medicine and Dentistry 20 43%
Neuroscience 2 4%
Biochemistry, Genetics and Molecular Biology 2 4%
Agricultural and Biological Sciences 2 4%
Immunology and Microbiology 1 2%
Other 6 13%
Unknown 13 28%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 03 November 2015.
All research outputs
#12,510,835
of 15,747,062 outputs
Outputs from Journal of Applied Oral Science
#138
of 220 outputs
Outputs of similar age
#196,058
of 287,465 outputs
Outputs of similar age from Journal of Applied Oral Science
#1
of 1 outputs
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So far Altmetric has tracked 220 research outputs from this source. They receive a mean Attention Score of 2.9. This one is in the 19th percentile – i.e., 19% of its peers scored the same or lower than it.
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