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Chapter title |
Application of Higher Density Iron Oxide Nanoparticle Pellicles to Enrich the Plasma Membrane and Its Proteome from Cells in Suspension
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Chapter number | 5 |
Book title |
The Surfaceome
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Published in |
Methods in molecular biology, January 2018
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DOI | 10.1007/978-1-4939-7553-2_5 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7551-8, 978-1-4939-7553-2
|
Authors |
Rebecca L. Rose, Waeowalee Choksawangkarn, Catherine Fenselau |
Abstract |
Enrichment of the plasma membrane represents one valuable method to characterize the surfaceome, along with other plasma membrane and structural proteins. Currently, the overlapping densities of many subcellular organelles hinder enrichment of the plasma membrane by centrifugation. However, external access to the plasma membrane of intact cells allows the attachment of a nanoparticle pellicle to enhance its density and facilitate enrichment. We describe the synthesis of iron oxide nanoparticles, attachment of the pellicle to suspended cells, and recovery of plasma membrane proteins for proteomic analysis. |
Mendeley readers
The data shown below were compiled from readership statistics for 5 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 5 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Unspecified | 1 | 20% |
Student > Ph. D. Student | 1 | 20% |
Professor > Associate Professor | 1 | 20% |
Student > Bachelor | 1 | 20% |
Researcher | 1 | 20% |
Other | 0 | 0% |
Readers by discipline | Count | As % |
---|---|---|
Unspecified | 1 | 20% |
Pharmacology, Toxicology and Pharmaceutical Science | 1 | 20% |
Biochemistry, Genetics and Molecular Biology | 1 | 20% |
Agricultural and Biological Sciences | 1 | 20% |
Unknown | 1 | 20% |