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Mendeley readers
Chapter title |
Detecting Cell Surface Expression of the G Protein-Coupled Receptor CXCR4
|
---|---|
Chapter number | 10 |
Book title |
The Surfaceome
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Published in |
Methods in molecular biology, January 2018
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DOI | 10.1007/978-1-4939-7553-2_10 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7551-8, 978-1-4939-7553-2
|
Authors |
Amanda M. Nevins, Adriano Marchese |
Abstract |
G protein-coupled receptors (GPCRs) are cell surface receptors that relay extracellular signals to the inside of the cells. C-X-C chemokine receptor 4 (CXCR4) is a GPCR that undergoes receptor internalization and recycling upon stimulation with its cognate ligand, C-X-C chemokine 12 (CXCL12). Using this receptor/ligand pair we describe the use of two techniques, enzyme-linked immunosorbent assay (ELISA) and flow cytometry, widely used to quantify GPCR internalization from the plasma membrane and its return to the cell surface by recycling. |
Mendeley readers
The data shown below were compiled from readership statistics for 9 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 9 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Bachelor | 3 | 33% |
Researcher | 2 | 22% |
Student > Doctoral Student | 1 | 11% |
Student > Ph. D. Student | 1 | 11% |
Other | 1 | 11% |
Other | 0 | 0% |
Unknown | 1 | 11% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 5 | 56% |
Nursing and Health Professions | 1 | 11% |
Business, Management and Accounting | 1 | 11% |
Agricultural and Biological Sciences | 1 | 11% |
Unknown | 1 | 11% |