↓ Skip to main content

Novel monoclonal antibodies to study tissue regeneration in planarians

Overview of attention for article published in BMC Developmental Biology, January 2015
Altmetric Badge

About this Attention Score

  • In the top 25% of all research outputs scored by Altmetric
  • Among the highest-scoring outputs from this source (#16 of 207)
  • High Attention Score compared to outputs of the same age (86th percentile)
  • High Attention Score compared to outputs of the same age and source (93rd percentile)

Mentioned by

blogs
1 blog
twitter
2 tweeters

Citations

dimensions_citation
35 Dimensions

Readers on

mendeley
68 Mendeley
You are seeing a free-to-access but limited selection of the activity Altmetric has collected about this research output. Click here to find out more.
Title
Novel monoclonal antibodies to study tissue regeneration in planarians
Published in
BMC Developmental Biology, January 2015
DOI 10.1186/s12861-014-0050-9
Pubmed ID
Authors

Kelly G Ross, Kerilyn C Omuro, Matthew R Taylor, Roma K Munday, Amy Hubert, Ryan S King, Ricardo M Zayas

Abstract

BackgroundPlanarians are an attractive model organism for studying stem cell-based regeneration due to their ability to replace all of their tissues from a population of adult stem cells. The molecular toolkit for planarian studies currently includes the ability to study gene function using RNA interference (RNAi) and observe gene expression via in situ hybridizations. However, there are few antibodies available to visualize protein expression, which would greatly enhance analysis of RNAi experiments as well as allow further characterization of planarian cell populations using immunocytochemistry and other immunological techniques. Thus, additional, easy-to-use, and widely available monoclonal antibodies would be advantageous to study regeneration in planarians.ResultsWe have created seven monoclonal antibodies by inoculating mice with formaldehyde-fixed cells isolated from dissociated 3-day regeneration blastemas. These monoclonal antibodies can be used to label muscle fibers, axonal projections in the central and peripheral nervous systems, two populations of intestinal cells, ciliated cells, a subset of neoblast progeny, and discrete cells within the central nervous system as well as the regeneration blastema. We have tested these antibodies using eight variations of a formaldehyde-based fixation protocol and determined reliable protocols for immunolabeling whole planarians with each antibody. We found that labeling efficiency for each antibody varies greatly depending on the addition or removal of tissue processing steps that are commonly used for in situ hybridization or immunolabeling techniques. Our experiments show that a subset of the antibodies can be used alongside markers commonly used in planarian research, including anti-SYNAPSIN and anti-SMEDWI, or following whole-mount in situ hybridization experiments.ConclusionsThe monoclonal antibodies described in this paper will be a valuable resource for planarian research. These antibodies have the potential to be used to better understand planarian biology and to characterize phenotypes following RNAi experiments. In addition, we present alterations to fixation protocols and demonstrate how these changes can increase the labeling efficiencies of antibodies used to stain whole planarians.

Twitter Demographics

The data shown below were collected from the profiles of 2 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 68 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United Kingdom 2 3%
Spain 1 1%
United States 1 1%
Germany 1 1%
Unknown 63 93%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 18 26%
Student > Bachelor 11 16%
Researcher 6 9%
Student > Postgraduate 5 7%
Professor > Associate Professor 5 7%
Other 14 21%
Unknown 9 13%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 27 40%
Agricultural and Biological Sciences 26 38%
Engineering 2 3%
Physics and Astronomy 1 1%
Neuroscience 1 1%
Other 1 1%
Unknown 10 15%

Attention Score in Context

This research output has an Altmetric Attention Score of 10. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 27 January 2016.
All research outputs
#723,429
of 7,054,473 outputs
Outputs from BMC Developmental Biology
#16
of 207 outputs
Outputs of similar age
#30,712
of 231,121 outputs
Outputs of similar age from BMC Developmental Biology
#1
of 15 outputs
Altmetric has tracked 7,054,473 research outputs across all sources so far. Compared to these this one has done well and is in the 89th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 207 research outputs from this source. They receive a mean Attention Score of 3.2. This one has done particularly well, scoring higher than 91% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 231,121 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 86% of its contemporaries.
We're also able to compare this research output to 15 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 93% of its contemporaries.