Usage of a Bioluminescence Reporter System to Image Promoter Activity During Host Infection
Methods in molecular biology, January 2018
Gili Aviv, Ohad Gal-Mor
Bioluminescence is the process of production and emission of light by a living organism, usually as the by-product of the oxidative enzyme, luciferase. Currently available technology allows for the exploitation of a bioluminescent reporter system to study bacterial gene regulation during rodent infection, in real time, over a large dynamic range. Here we show how this imaging system can be used to study virulence gene regulation during Salmonella enterica infection in the mouse model. To demonstrate this technique we show the ex vivo expression pattern of the gene dksA, encoding a conserved and pleotropic regulator, which plays a key role in Salmonella pathogenicity .
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