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Host-Pathogen Interactions

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Cover of 'Host-Pathogen Interactions'

Table of Contents

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    Book Overview
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    Chapter 1 Genetic Association Studies in Host–Pathogen Interaction Analysis
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    Chapter 2 Bacterial Genotyping Methods: From the Basics to Modern
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    Chapter 3 Real-Time Reverse Transcription PCR as a Tool to Study Virulence Gene Regulation in Bacterial Pathogens
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    Chapter 4 Usage of a Bioluminescence Reporter System to Image Promoter Activity During Host Infection
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    Chapter 5 lacZ Reporter System as a Tool to Study Virulence Gene Regulation in Bacterial Pathogens
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    Chapter 6 Western Blotting Against Tagged Virulence Determinants to Study Bacterial Pathogenicity
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    Chapter 7 Molecular Methods to Analyze the Effect of Proteins Expressed by Salmonella During Its Intracellular Stage
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    Chapter 8 Organoids as a Model to Study Infectious Disease
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    Chapter 9 Surface Proteome Biotinylation Combined with Bioinformatic Tools as a Strategy for Predicting Pathogen Interacting Proteins
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    Chapter 10 Systems Biology Modeling to Study Pathogen–Host Interactions
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    Chapter 11 Phage Therapy: Various Perspectives on How to Improve the Art
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    Chapter 12 Application of RNA-seq and Bioimaging Methods to Study Microbe–Microbe Interactions and Their Effects on Biofilm Formation and Gene Expression
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    Chapter 13 Serial Dilution-Based Growth Curves and Growth Curve Synchronization for High-Resolution Time Series of Bacterial Biofilm Growth
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    Chapter 14 Detection of Bacterial Quorum Sensing Molecules
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    Chapter 15 Generating Chromosome-Located Transcriptional Fusions to Fluorescent Proteins for Single-Cell Gene Expression Analysis in Pseudomonas syringae
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    Chapter 16 Introduction of Genetic Material in Ralstonia solanacearum Through Natural Transformation and Conjugation
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    Chapter 17 In Vitro and In Vivo Secretion/Translocation Assays to Identify Novel Ralstonia solanacearum Type 3 Effectors
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    Chapter 18 Plant Pathogenicity Phenotyping of Ralstonia solanacearum Strains
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    Chapter 19 Methods to Quantify Biotic-Induced Stress in Plants
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    Chapter 20 From Sample to Data: Preparing, Obtaining, and Analyzing Images of Plant-Pathogen Interactions Using Confocal Microscopy
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    Chapter 21 Screening of c-di-GMP-Regulated Exopolysaccharides in Host Interacting Bacteria
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    Chapter 22 Primary Characterization of Small RNAs in Symbiotic Nitrogen-Fixing Bacteria
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    Chapter 23 A New, Nondestructive, Split-Root System for Local and Systemic Plant Responses Studies with Soybean
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    Chapter 24 Methods for the Characterization of Plant-Growth Promoting Rhizobacteria
Attention for Chapter 8: Organoids as a Model to Study Infectious Disease
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Chapter title
Organoids as a Model to Study Infectious Disease
Chapter number 8
Book title
Host-Pathogen Interactions
Published in
Methods in molecular biology, January 2018
DOI 10.1007/978-1-4939-7604-1_8
Pubmed ID
Book ISBNs
978-1-4939-7603-4, 978-1-4939-7604-1
Authors

Kristen A. Engevik, Andrea L. Matthis, Marshall H. Montrose, Eitaro Aihara

Abstract

The advent of the gastric organoid culture system has provided a new model to emulate native epithelial tissue in vitro. Gastric organoids grow from isolated epithelial stem cells and develop into three dimensional structures that can be used to study host physiology. Here we describe current laboratory protocols for growing gastric organoids and the microinjection of pathogens such as Helicobacter pylori into the lumen of gastric organoids in order to study the cellular response following infection.

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X Demographics

The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 25 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 25 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 8 32%
Student > Bachelor 4 16%
Researcher 3 12%
Student > Doctoral Student 1 4%
Professor 1 4%
Other 4 16%
Unknown 4 16%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 6 24%
Agricultural and Biological Sciences 6 24%
Immunology and Microbiology 4 16%
Medicine and Dentistry 3 12%
Computer Science 1 4%
Other 2 8%
Unknown 3 12%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 08 January 2018.
All research outputs
#19,594,120
of 24,093,053 outputs
Outputs from Methods in molecular biology
#8,407
of 13,601 outputs
Outputs of similar age
#338,972
of 449,849 outputs
Outputs of similar age from Methods in molecular biology
#942
of 1,480 outputs
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So far Altmetric has tracked 13,601 research outputs from this source. They receive a mean Attention Score of 3.5. This one is in the 23rd percentile – i.e., 23% of its peers scored the same or lower than it.
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