Chapter title |
Primary Characterization of Small RNAs in Symbiotic Nitrogen-Fixing Bacteria
|
---|---|
Chapter number | 22 |
Book title |
Host-Pathogen Interactions
|
Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-7604-1_22 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7603-4, 978-1-4939-7604-1
|
Authors |
Marta Robledo, Natalia I. García-Tomsig, José I. Jiménez-Zurdo |
Abstract |
High-throughput transcriptome profiling (RNAseq) has uncovered large and heterogeneous populations of small noncoding RNA species (sRNAs) with potential regulatory roles in bacteria. A large fraction of sRNAs are differentially regulated and rely on protein-assisted antisense interactions to trans-encoded target mRNAs to fine-tune posttranscriptional reprogramming of gene expression in response to external cues. However, annotation and function of sRNAs are still largely overlooked in nonmodel bacteria with complex lifestyles. Here, we describe experimental protocols successfully applied for the accurate annotation, expression profiling and target mRNA identification of trans-acting sRNAs in the nitrogen-fixing α-rhizobium Sinorhizobium meliloti. The protocols presented here can be similarly applied for the characterization of trans-sRNAs in genetically tractable α-proteobacteria of agronomical or clinical relevance interacting with eukaryotic hosts. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 3 | 100% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 3 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 7 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 3 | 43% |
Student > Ph. D. Student | 1 | 14% |
Student > Postgraduate | 1 | 14% |
Other | 1 | 14% |
Unknown | 1 | 14% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 2 | 29% |
Nursing and Health Professions | 1 | 14% |
Agricultural and Biological Sciences | 1 | 14% |
Medicine and Dentistry | 1 | 14% |
Unknown | 2 | 29% |