Chapter title |
Real-Time Reverse Transcription PCR as a Tool to Study Virulence Gene Regulation in Bacterial Pathogens
|
---|---|
Chapter number | 3 |
Book title |
Host-Pathogen Interactions
|
Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-7604-1_3 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7603-4, 978-1-4939-7604-1
|
Authors |
Gili Aviv, Ohad Gal-Mor |
Abstract |
Quantitative real-time PCR (qRT-PCR) is a highly sensitive and reliable method for detection and quantification of DNA. When combined with a prior stage of RNA reverse transcription to generate complementary DNA (cDNA), this is a powerful approach to determine and analyze gene transcriptional expression. Real-time quantitative reverse transcription PCR has become the gold standard method in studying genes expression and virulence regulation under various genetic backgrounds (e.g., in the absence of regulators) or environmental conditions. Here we demonstrate the utilization of this approach to study the transcriptional regulation of the conjugation pilus of the Salmonella enterica serovar Infantis virulence plasmid (pESI). |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 16 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Researcher | 4 | 25% |
Student > Ph. D. Student | 4 | 25% |
Student > Doctoral Student | 2 | 13% |
Student > Bachelor | 1 | 6% |
Student > Master | 1 | 6% |
Other | 1 | 6% |
Unknown | 3 | 19% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 4 | 25% |
Agricultural and Biological Sciences | 4 | 25% |
Nursing and Health Professions | 1 | 6% |
Chemistry | 1 | 6% |
Unknown | 6 | 38% |