Chapter title |
Application of RNA-seq and Bioimaging Methods to Study Microbe–Microbe Interactions and Their Effects on Biofilm Formation and Gene Expression
|
---|---|
Chapter number | 12 |
Book title |
Host-Pathogen Interactions
|
Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-7604-1_12 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7603-4, 978-1-4939-7604-1
|
Authors |
Cristina Isabel Amador, Claus Sternberg, Lars Jelsbak |
Abstract |
Complex interactions between pathogenic bacteria, the microbiota, and the host can modify pathogen physiology and behavior. We describe two different experimental approaches to study microbe-microbe interactions in in vitro systems containing surface-associated microbial populations. One method is the application of RNA sequencing (RNA-seq) to determine the transcriptional changes in pathogenic bacteria in response to microbial interspecies interactions. The other method combines flow cell devices for bacterial cultivation and growth with high-resolution bioimaging to analyze the microscale structural organization of interacting microbial populations within mixed-species biofilms. |
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United States | 3 | 30% |
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Unknown | 6 | 60% |
Demographic breakdown
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Members of the public | 6 | 60% |
Scientists | 4 | 40% |
Mendeley readers
Geographical breakdown
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Unknown | 25 | 100% |
Demographic breakdown
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Student > Ph. D. Student | 6 | 24% |
Student > Bachelor | 5 | 20% |
Researcher | 4 | 16% |
Professor | 2 | 8% |
Other | 1 | 4% |
Other | 2 | 8% |
Unknown | 5 | 20% |
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Engineering | 2 | 8% |
Pharmacology, Toxicology and Pharmaceutical Science | 1 | 4% |
Immunology and Microbiology | 1 | 4% |
Other | 3 | 12% |
Unknown | 6 | 24% |