Title |
Categorization of multiple sclerosis relapse subtypes by B cell profiling in the blood
|
---|---|
Published in |
Acta Neuropathologica Communications, September 2014
|
DOI | 10.1186/s40478-014-0138-2 |
Pubmed ID | |
Authors |
Christopher Hohmann, Bianca Milles, Michael Schinke, Michael Schroeter, Jochen Ulzheimer, Peter Kraft, Christoph Kleinschnitz, Paul V Lehmann, Stefanie Kuerten |
Abstract |
B cells are attracting increasing attention in the pathogenesis of multiple sclerosis (MS). B cell-targeted therapies with monoclonal antibodies or plasmapheresis have been shown to be successful in a subset of patients. Here, patients with either relapsing-remitting (n = 24) or secondary progressive (n = 6) MS presenting with an acute clinical relapse were screened for their B cell reactivity to brain antigens and were re-tested three to nine months later. Enzyme-linked immunospot technique (ELISPOT) was used to identify brain-reactive B cells in peripheral blood mononuclear cells (PBMC) directly ex vivo and after 96 h of polyclonal stimulation. Clinical severity of symptoms was determined using the Expanded Disability Status Scale (EDSS). |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 38 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 6 | 16% |
Student > Doctoral Student | 5 | 13% |
Student > Master | 5 | 13% |
Student > Ph. D. Student | 4 | 11% |
Student > Postgraduate | 4 | 11% |
Other | 9 | 24% |
Unknown | 5 | 13% |
Readers by discipline | Count | As % |
---|---|---|
Medicine and Dentistry | 14 | 37% |
Agricultural and Biological Sciences | 6 | 16% |
Neuroscience | 3 | 8% |
Immunology and Microbiology | 3 | 8% |
Biochemistry, Genetics and Molecular Biology | 2 | 5% |
Other | 3 | 8% |
Unknown | 7 | 18% |