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DNA Topoisomerases

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Cover of 'DNA Topoisomerases'

Table of Contents

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    Book Overview
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    Chapter 1 Type IA DNA Topoisomerases: A Universal Core and Multiple Activities
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    Chapter 2 Topoisomerase I and Genome Stability: The Good and the Bad
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    Chapter 3 DNA Topoisomerases as Targets for Antibacterial Agents
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    Chapter 4 DNA Supercoiling Measurement in Bacteria
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    Chapter 5 DNA Catenation Reveals the Dynamics of DNA Topology During Replication
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    Chapter 6 Mapping E. coli Topoisomerase IV Binding and Activity Sites
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    Chapter 7 The Use of Psoralen Photobinding to Study Transcription-Induced Supercoiling
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    Chapter 8 Immunoprecipitation of RNA:DNA Hybrids from Budding Yeast
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    Chapter 9 Detection of oriC-Independent Replication in Escherichia coli Cells
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    Chapter 10 Single-Molecule Magnetic Tweezer Analysis of Topoisomerases
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    Chapter 11 Synthesis of Hemicatenanes for the Study of Type IA Topoisomerases
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    Chapter 12 An Assay for Detecting RNA Topoisomerase Activity
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    Chapter 13 Studying TDP1 Function in DNA Repair
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    Chapter 14 Topoisomerase II Chromatin Immunoprecipitation
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    Chapter 15 Analyzing Mitotic Chromosome Structural Defects After Topoisomerase II Inhibition or Mutation
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    Chapter 16 Monitoring the DNA Topoisomerase II Checkpoint in Saccharomyces cerevisiae
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    Chapter 17 Studying Topoisomerase 1-Mediated Damage at Genomic Ribonucleotides
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    Chapter 18 A Fluorescence-Based Assay for Identification of Bacterial Topoisomerase I Poisons
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    Chapter 19 Fluoroquinolone-Gyrase-DNA Cleaved Complexes
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    Chapter 20 Detection of Topoisomerase Covalent Complexes in Eukaryotic Cells
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    Chapter 21 Visualization and Quantification of Topoisomerase–DNA Covalent Complexes Using the Trapped in Agarose Immunostaining (TARDIS) Assay
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    Chapter 22 Study of Plasmid-Mediated Quinolone Resistance in Bacteria
Attention for Chapter 6: Mapping E. coli Topoisomerase IV Binding and Activity Sites
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Chapter title
Mapping E. coli Topoisomerase IV Binding and Activity Sites
Chapter number 6
Book title
DNA Topoisomerases
Published in
Methods in molecular biology, January 2018
DOI 10.1007/978-1-4939-7459-7_6
Pubmed ID
Book ISBNs
978-1-4939-7458-0, 978-1-4939-7459-7
Authors

Hafez El Sayyed, Olivier Espéli

Abstract

This methods article described a protocol aiming at mapping E. coli Topoisomerase IV (Topo IV) binding and cleavage activity sites on the genome. The approach is readily applicable to any Type II topoisomerase on a broad variety of gram-positive and gram-negative bacterial species. Conventional ChIP-seq of flag tagged Topo IV subunits and a novel method aimed at trapping only DNA bound to active Topo IV (called NorfliP) are described. NorfliP relies on the ability of norfloxacin, a quinolone drug, to cross-link the 5' ends of the DNA breaks with the catalytic tyrosine of bacterial Type II topoisomerases. These methods give complementary results and their combination brought important insights on both the function and regulation of Topo IV.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 12 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 12 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 5 42%
Other 1 8%
Student > Bachelor 1 8%
Student > Master 1 8%
Researcher 1 8%
Other 1 8%
Unknown 2 17%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 6 50%
Chemical Engineering 1 8%
Nursing and Health Professions 1 8%
Medicine and Dentistry 1 8%
Unknown 3 25%