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DNA Topoisomerases

Overview of attention for book
Cover of 'DNA Topoisomerases'

Table of Contents

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    Book Overview
  2. Altmetric Badge
    Chapter 1 Type IA DNA Topoisomerases: A Universal Core and Multiple Activities
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    Chapter 2 Topoisomerase I and Genome Stability: The Good and the Bad
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    Chapter 3 DNA Topoisomerases as Targets for Antibacterial Agents
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    Chapter 4 DNA Supercoiling Measurement in Bacteria
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    Chapter 5 DNA Catenation Reveals the Dynamics of DNA Topology During Replication
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    Chapter 6 Mapping E. coli Topoisomerase IV Binding and Activity Sites
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    Chapter 7 The Use of Psoralen Photobinding to Study Transcription-Induced Supercoiling
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    Chapter 8 Immunoprecipitation of RNA:DNA Hybrids from Budding Yeast
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    Chapter 9 Detection of oriC-Independent Replication in Escherichia coli Cells
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    Chapter 10 Single-Molecule Magnetic Tweezer Analysis of Topoisomerases
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    Chapter 11 Synthesis of Hemicatenanes for the Study of Type IA Topoisomerases
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    Chapter 12 An Assay for Detecting RNA Topoisomerase Activity
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    Chapter 13 Studying TDP1 Function in DNA Repair
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    Chapter 14 Topoisomerase II Chromatin Immunoprecipitation
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    Chapter 15 Analyzing Mitotic Chromosome Structural Defects After Topoisomerase II Inhibition or Mutation
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    Chapter 16 Monitoring the DNA Topoisomerase II Checkpoint in Saccharomyces cerevisiae
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    Chapter 17 Studying Topoisomerase 1-Mediated Damage at Genomic Ribonucleotides
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    Chapter 18 A Fluorescence-Based Assay for Identification of Bacterial Topoisomerase I Poisons
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    Chapter 19 Fluoroquinolone-Gyrase-DNA Cleaved Complexes
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    Chapter 20 Detection of Topoisomerase Covalent Complexes in Eukaryotic Cells
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    Chapter 21 Visualization and Quantification of Topoisomerase–DNA Covalent Complexes Using the Trapped in Agarose Immunostaining (TARDIS) Assay
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    Chapter 22 Study of Plasmid-Mediated Quinolone Resistance in Bacteria
Attention for Chapter 10: Single-Molecule Magnetic Tweezer Analysis of Topoisomerases
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Chapter title
Single-Molecule Magnetic Tweezer Analysis of Topoisomerases
Chapter number 10
Book title
DNA Topoisomerases
Published in
Methods in molecular biology, January 2018
DOI 10.1007/978-1-4939-7459-7_10
Pubmed ID
Book ISBNs
978-1-4939-7458-0, 978-1-4939-7459-7
Authors

Kathryn H. Gunn, John F. Marko, Alfonso Mondragón

Abstract

Magnetic tweezers (MT) provide a powerful single-molecule approach to study the mechanism of topoisomerases, giving the experimenter the ability to change and read out DNA topology in real time. By using diverse DNA substrates, one can study different aspects of topoisomerase function and arrive at a better mechanistic understanding of these fascinating enzymes. Here we describe methods for the creation of three different DNA substrates used in MT experiments with topoisomerases: double-stranded DNA (dsDNA) tethers, "braided" (intertwined or catenated) DNA tether pairs, and dsDNA tethers with single-stranded DNA (ssDNA) regions. Additionally, we discuss how to build flow cells for bright-field MT microscopy, as well as how to noncovalently attach anti-digoxigenin to the coverslip surface for tethering digoxigenin-labeled DNAs. Finally, we describe procedures for the identification of a suitable DNA substrate for MT study and data collection.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 11 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 11 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 3 27%
Student > Postgraduate 2 18%
Unspecified 1 9%
Student > Master 1 9%
Student > Bachelor 1 9%
Other 2 18%
Unknown 1 9%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 5 45%
Unspecified 1 9%
Agricultural and Biological Sciences 1 9%
Neuroscience 1 9%
Engineering 1 9%
Other 0 0%
Unknown 2 18%