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Pyrosequencing

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Cover of 'Pyrosequencing'

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter 1 The History of Pyrosequencing ®
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    Chapter 2 PyroMark(®) Instruments, Chemistry, and Software for Pyrosequencing(®) Analysis.
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    Chapter 3 Software-Based Pyrogram ® Evaluation
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    Chapter 4 Quantitative Validation and Quality Control of Pyrosequencing ® Assays
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    Chapter 5 Extended KRAS and NRAS Mutation Profiling by Pyrosequencing ®
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    Chapter 6 Universal BRAF State Detection by the Pyrosequencing ® -Based U-BRAF V600 Assay
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    Chapter 7 Pyrosequencing
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    Chapter 8 Analysis of Mutational Hotspots in Routinely Processed Bone Marrow Trephines by Pyrosequencing ®
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    Chapter 9 Analysis of Copy Number Variation by Pyrosequencing(®) Using Paralogous Sequences.
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    Chapter 10 Prenatal Diagnosis of Chromosomal Aneuploidies by Quantitative Pyrosequencing(®).
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    Chapter 11 HLA-B and HLA-C Supratyping by Pyrosequencing ®
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    Chapter 12 Allele Quantification Pyrosequencing(®) at Designated SNP Sites to Detect Allelic Expression Imbalance and Loss-of-Heterozygosity.
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    Chapter 13 Quantitative DNA Methylation Analysis by Pyrosequencing(®).
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    Chapter 14 Quantitative Methylation Analysis of the PCDHB Gene Cluster.
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    Chapter 15 Assessment of Changes in Global DNA Methylation Levels by Pyrosequencing(®) of Repetitive Elements.
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    Chapter 16 Global Analysis of DNA 5-Methylcytosine Using the Luminometric Methylation Assay, LUMA.
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    Chapter 17 Limiting Dilution Bisulfite Pyrosequencing(®): A Method for Methylation Analysis of Individual DNA Molecules in a Single or a Few Cells.
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    Chapter 18 Detection of Loss of Imprinting by Pyrosequencing(®).
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    Chapter 19 Analysis of DNA Methylation Patterns in Single Blastocysts by Pyrosequencing(®).
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    Chapter 20 Allele-Specific DNA Methylation Detection by Pyrosequencing(®).
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    Chapter 21 SNP-Based Quantification of Allele-Specific DNA Methylation Patterns by Pyrosequencing ®
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    Chapter 22 DNA Methylation Analysis of ChIP Products at Single Nucleotide Resolution by Pyrosequencing(®).
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    Chapter 23 Multiplex Pyrosequencing ® : Simultaneous Genotyping Based on SNPs from Distant Genomic Regions
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    Chapter 24 Pyrosequencing
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    Chapter 25 Application of Pyrosequencing ® in Food Biodefense
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    Chapter 26 Pyrosequencing
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    Chapter 27 Tissue-Specific DNA Methylation Patterns in Forensic Samples Detected by Pyrosequencing(®).
Attention for Chapter 9: Analysis of Copy Number Variation by Pyrosequencing(®) Using Paralogous Sequences.
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Chapter title
Analysis of Copy Number Variation by Pyrosequencing(®) Using Paralogous Sequences.
Chapter number 9
Book title
Pyrosequencing
Published in
Methods in molecular biology, January 2015
DOI 10.1007/978-1-4939-2715-9_9
Pubmed ID
Book ISBNs
978-1-4939-2714-2, 978-1-4939-2715-9
Authors

Kringen, Marianne Kristiansen, Marianne Kristiansen Kringen

Abstract

The determination of gene copy numbers of highly similar genes is difficult with conventional PCR-based methods. However, by amplification of similar genes in the same PCR reaction followed by Pyrosequencing(®), one may distinguish the genes based on a single-nucleotide difference. The ratio between the peak heights of gene-specific nucleotides obtained in the Pyrosequencing reaction may thereby be used to calculate the relative copy numbers of target genes. This method is easy and cost effective compared to other methods, and allows for the determination of copy numbers of genes that were previously difficult to achieve.

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Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 25 June 2015.
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#18,417,643
of 22,815,414 outputs
Outputs from Methods in molecular biology
#7,914
of 13,120 outputs
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#255,858
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Outputs of similar age from Methods in molecular biology
#481
of 997 outputs
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