Chapter title |
Allele-Specific DNA Methylation Detection by Pyrosequencing(®).
|
---|---|
Chapter number | 20 |
Book title |
Pyrosequencing
|
Published in |
Methods in molecular biology, January 2015
|
DOI | 10.1007/978-1-4939-2715-9_20 |
Pubmed ID | |
Book ISBNs |
978-1-4939-2714-2, 978-1-4939-2715-9
|
Authors |
Kristensen, Lasse Sommer, Johansen, Jens Vilstrup, Grønbæk, Kirsten, Lasse Sommer Kristensen, Jens Vilstrup Johansen, Kirsten Grønbæk |
Abstract |
DNA methylation is an epigenetic modification that plays important roles in healthy as well as diseased cells, by influencing the transcription of genes. In spite the fact that human somatic cells are diploid, most of the currently available methods for the study of DNA methylation do not provide information on the methylation status of individual alleles of genes. This information may be of importance in many situations. In particular, in cancer both alleles of tumour suppressor genes generally need to be inactivated for a phenotypic effect to be observed. Here, we present a simple and cost-effective protocol for allele-specific DNA methylation detection based on Pyrosequencing(®) of methylation-specific PCR (MSP) products including a single nucleotide polymorphism (SNP) within the amplicon. |
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