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Pyrosequencing

Overview of attention for book
Cover of 'Pyrosequencing'

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter 1 The History of Pyrosequencing ®
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    Chapter 2 PyroMark(®) Instruments, Chemistry, and Software for Pyrosequencing(®) Analysis.
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    Chapter 3 Software-Based Pyrogram ® Evaluation
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    Chapter 4 Quantitative Validation and Quality Control of Pyrosequencing ® Assays
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    Chapter 5 Extended KRAS and NRAS Mutation Profiling by Pyrosequencing ®
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    Chapter 6 Universal BRAF State Detection by the Pyrosequencing ® -Based U-BRAF V600 Assay
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    Chapter 7 Pyrosequencing
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    Chapter 8 Analysis of Mutational Hotspots in Routinely Processed Bone Marrow Trephines by Pyrosequencing ®
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    Chapter 9 Analysis of Copy Number Variation by Pyrosequencing(®) Using Paralogous Sequences.
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    Chapter 10 Prenatal Diagnosis of Chromosomal Aneuploidies by Quantitative Pyrosequencing(®).
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    Chapter 11 HLA-B and HLA-C Supratyping by Pyrosequencing ®
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    Chapter 12 Allele Quantification Pyrosequencing(®) at Designated SNP Sites to Detect Allelic Expression Imbalance and Loss-of-Heterozygosity.
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    Chapter 13 Quantitative DNA Methylation Analysis by Pyrosequencing(®).
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    Chapter 14 Quantitative Methylation Analysis of the PCDHB Gene Cluster.
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    Chapter 15 Assessment of Changes in Global DNA Methylation Levels by Pyrosequencing(®) of Repetitive Elements.
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    Chapter 16 Global Analysis of DNA 5-Methylcytosine Using the Luminometric Methylation Assay, LUMA.
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    Chapter 17 Limiting Dilution Bisulfite Pyrosequencing(®): A Method for Methylation Analysis of Individual DNA Molecules in a Single or a Few Cells.
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    Chapter 18 Detection of Loss of Imprinting by Pyrosequencing(®).
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    Chapter 19 Analysis of DNA Methylation Patterns in Single Blastocysts by Pyrosequencing(®).
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    Chapter 20 Allele-Specific DNA Methylation Detection by Pyrosequencing(®).
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    Chapter 21 SNP-Based Quantification of Allele-Specific DNA Methylation Patterns by Pyrosequencing ®
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    Chapter 22 DNA Methylation Analysis of ChIP Products at Single Nucleotide Resolution by Pyrosequencing(®).
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    Chapter 23 Multiplex Pyrosequencing ® : Simultaneous Genotyping Based on SNPs from Distant Genomic Regions
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    Chapter 24 Pyrosequencing
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    Chapter 25 Application of Pyrosequencing ® in Food Biodefense
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    Chapter 26 Pyrosequencing
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    Chapter 27 Tissue-Specific DNA Methylation Patterns in Forensic Samples Detected by Pyrosequencing(®).
Attention for Chapter 2: PyroMark(®) Instruments, Chemistry, and Software for Pyrosequencing(®) Analysis.
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Chapter title
PyroMark(®) Instruments, Chemistry, and Software for Pyrosequencing(®) Analysis.
Chapter number 2
Book title
Pyrosequencing
Published in
Methods in molecular biology, January 2015
DOI 10.1007/978-1-4939-2715-9_2
Pubmed ID
26103888
Book ISBNs
978-1-4939-2714-2, 978-1-4939-2715-9
Authors

Kreutz, Martin, Schock, Gerald, Kaiser, Julia, Hochstein, Norbert, Peist, Ralf, Martin Kreutz, Gerald Schock, Julia Kaiser, Norbert Hochstein, Ralf Peist

Abstract

Since the early 2000s, Pyrosequencing(®) technology has been adapted for various instrument platforms to enable users to examine the role of epigenetic DNA methylation in gene expression regulation, genetic markers for specific phenotypes in livestock, drug resistance development in pathogens, and polymorphisms in forensic samples of mitochondrial DNA.The instruments, software, and chemistry have been modified to facilitate different sample throughputs and sample amounts. Just recently, major changes have been implemented to enable increased read length and more precise Pyrosequencing results. These improvements were made possible through a number of changes to various system components. In addition, assay development has been streamlined through the availability of optimized PCR and Pyrosequencing reagents, automated assay design tools, and a number of predesigned Pyrosequencing assays.In future, instruments with smaller footprints and the ability to automate crucial steps of the Pyrosequencing protocol will be available and will provide even more convenient and standardized Pyrosequencing analysis with flexible throughput.

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X Demographics

The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
 Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 13 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 13 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 2 15%
Lecturer > Senior Lecturer 1 8%
Student > Bachelor 1 8%
Student > Master 1 8%
Professor 1 8%
Other 2 15%
Unknown 5 38%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 2 15%
Agricultural and Biological Sciences 2 15%
Computer Science 1 8%
Environmental Science 1 8%
Unknown 7 54%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 25 June 2015.
All research outputs
#20,281,599
of 22,815,414 outputs
Outputs from Methods in molecular biology
#9,910
of 13,120 outputs
Outputs of similar age
#295,836
of 353,106 outputs
Outputs of similar age from Methods in molecular biology
#636
of 997 outputs
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So far Altmetric has tracked 13,120 research outputs from this source. They receive a mean Attention Score of 3.4. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
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We're also able to compare this research output to 997 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.

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