↓ Skip to main content

Discovery of a unique Ig heavy-chain isotype (IgT) in rainbow trout: Implications for a distinctive B cell developmental pathway in teleost fish

Overview of attention for article published in Proceedings of the National Academy of Sciences of the United States of America, April 2005
Altmetric Badge

About this Attention Score

  • Good Attention Score compared to outputs of the same age (66th percentile)
  • Average Attention Score compared to outputs of the same age and source

Mentioned by

wikipedia
1 Wikipedia page

Citations

dimensions_citation
286 Dimensions

Readers on

mendeley
132 Mendeley
Title
Discovery of a unique Ig heavy-chain isotype (IgT) in rainbow trout: Implications for a distinctive B cell developmental pathway in teleost fish
Published in
Proceedings of the National Academy of Sciences of the United States of America, April 2005
DOI 10.1073/pnas.0500027102
Pubmed ID
Authors

J. D. Hansen, E. D. Landis, R. B. Phillips

Abstract

During the analysis of Ig superfamily members within the available rainbow trout (Oncorhynchus mykiss) EST gene index, we identified a unique Ig heavy-chain (IgH) isotype. cDNAs encoding this isotype are composed of a typical IgH leader sequence and a VDJ rearranged segment followed by four Ig superfamily C-1 domains represented as either membrane-bound or secretory versions. Because teleost fish were previously thought to encode and express only two IgH isotypes (IgM and IgD) for their humoral immune repertoire, we isolated all three cDNA isotypes from a single homozygous trout (OSU-142) to confirm that all three are indeed independent isotypes. Bioinformatic and phylogenetic analysis indicates that this previously undescribed divergent isotype is restricted to bony fish, thus we have named this isotype "IgT" (tau) for teleost fish. Genomic sequence analysis of an OSU-142 bacterial artificial chromosome (BAC) clone positive for all three IgH isotypes revealed that IgT utilizes the standard rainbow trout V(H) families, but surprisingly, the IgT isotype possesses its own exclusive set of D(H) and J(H) elements for the generation of diversity. The IgT D and J segments and tau constant (C) region genes are located upstream of the D and J elements for IgM, representing a genomic IgH architecture that has not been observed in any other vertebrate class. All three isotypes are primarily expressed in the spleen and pronephros (bone marrow equivalent), and ontogenically, expression of IgT is present 4 d before hatching in developing embryos.

Mendeley readers

The data shown below were compiled from readership statistics for 132 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 2 2%
Spain 2 2%
United Kingdom 1 <1%
Mexico 1 <1%
France 1 <1%
Norway 1 <1%
India 1 <1%
Belgium 1 <1%
Austria 1 <1%
Other 0 0%
Unknown 121 92%

Demographic breakdown

Readers by professional status Count As %
Researcher 35 27%
Student > Ph. D. Student 33 25%
Student > Master 18 14%
Professor 9 7%
Unspecified 9 7%
Other 28 21%
Readers by discipline Count As %
Agricultural and Biological Sciences 84 64%
Unspecified 16 12%
Biochemistry, Genetics and Molecular Biology 11 8%
Immunology and Microbiology 9 7%
Medicine and Dentistry 5 4%
Other 7 5%

Attention Score in Context

This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 16 February 2015.
All research outputs
#3,552,495
of 12,363,980 outputs
Outputs from Proceedings of the National Academy of Sciences of the United States of America
#42,783
of 77,317 outputs
Outputs of similar age
#78,392
of 267,724 outputs
Outputs of similar age from Proceedings of the National Academy of Sciences of the United States of America
#636
of 979 outputs
Altmetric has tracked 12,363,980 research outputs across all sources so far. This one is in the 49th percentile – i.e., 49% of other outputs scored the same or lower than it.
So far Altmetric has tracked 77,317 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 22.1. This one is in the 17th percentile – i.e., 17% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 267,724 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 66% of its contemporaries.
We're also able to compare this research output to 979 others from the same source and published within six weeks on either side of this one. This one is in the 32nd percentile – i.e., 32% of its contemporaries scored the same or lower than it.