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X Demographics
Mendeley readers
| Title |
The Integrity and Yield of Genomic DNA Isolated from Whole Blood Following Long-Term Storage at −30°C
|
|---|---|
| Published in |
Biopreservation and Biobanking, January 2018
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| DOI | 10.1089/bio.2017.0050 |
| Pubmed ID | |
| Authors |
Wenlong Carl Chen, Robyn Kerr, Andrew May, Babongile Ndlovu, Anelisa Sobalisa, Sanelisiwe T. Duze, Lavania Joseph, Christopher G. Mathew, Chantal Babb de Villiers |
| Abstract |
Long-term storage of whole blood can affect the integrity of DNA if it is not done under optimal conditions. The aim of this study was to determine whether long-term storage (2-19 years) of whole blood samples at -30°C had a negative effect on the quality or quantity of genomic DNA that could be recovered at extraction. Genomic DNA was isolated from 2758 whole blood samples collected in 4 mL EDTA vacutainers from 1997 to 2012. DNA was extracted using the Qiagen® FlexiGene® DNA kit. The average storage duration at -30°C was 12 years. The quality and quantity of the isolated DNA were assessed using spectrophotometry (NanoDrop™), a fluorometric assay for double-stranded DNA (Qubit™), and agarose gel electrophoresis. The mean DNA yield per sample was found to be 114 μg from whole blood volumes that ranged from 0.5 to 4 mL. The mean A260/280 ratio and median A260/280 ratios were both 1.8. No correlation was found between the duration of storage and the total yield or the quality of DNA extracted. These data suggest that high-quality DNA can be extracted from whole blood samples that are stored at -30°C for up to 19 years. |
X Demographics
The data shown below were collected from the profiles of 11 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 11 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 10 | 91% |
| Scientists | 1 | 9% |
Mendeley readers
The data shown below were compiled from readership statistics for 59 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 59 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 9 | 15% |
| Researcher | 9 | 15% |
| Lecturer | 3 | 5% |
| Other | 3 | 5% |
| Student > Doctoral Student | 1 | 2% |
| Other | 4 | 7% |
| Unknown | 30 | 51% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 11 | 19% |
| Agricultural and Biological Sciences | 6 | 10% |
| Immunology and Microbiology | 2 | 3% |
| Chemistry | 2 | 3% |
| Nursing and Health Professions | 1 | 2% |
| Other | 4 | 7% |
| Unknown | 33 | 56% |