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The Integrity and Yield of Genomic DNA Isolated from Whole Blood Following Long-Term Storage at −30°C

Overview of attention for article published in Biopreservation and Biobanking, January 2018
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About this Attention Score

  • In the top 25% of all research outputs scored by Altmetric
  • Among the highest-scoring outputs from this source (#48 of 205)
  • Good Attention Score compared to outputs of the same age (74th percentile)
  • Above-average Attention Score compared to outputs of the same age and source (62nd percentile)

Mentioned by

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11 tweeters

Citations

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3 Dimensions

Readers on

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11 Mendeley
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Title
The Integrity and Yield of Genomic DNA Isolated from Whole Blood Following Long-Term Storage at −30°C
Published in
Biopreservation and Biobanking, January 2018
DOI 10.1089/bio.2017.0050
Pubmed ID
Authors

ChenWenlong Carl, KerrRobyn, MayAndrew, NdlovuBabongile, SobalisaAnelisa, DuzeSanelisiwe T., JosephLavania, MathewChristopher G., Babb de VilliersChantal

Abstract

Long-term storage of whole blood can affect the integrity of DNA if it is not done under optimal conditions. The aim of this study was to determine whether long-term storage (2-19 years) of whole blood samples at -30°C had a negative effect on the quality or quantity of genomic DNA that could be recovered at extraction. Genomic DNA was isolated from 2758 whole blood samples collected in 4 mL EDTA vacutainers from 1997 to 2012. DNA was extracted using the Qiagen® FlexiGene® DNA kit. The average storage duration at -30°C was 12 years. The quality and quantity of the isolated DNA were assessed using spectrophotometry (NanoDrop™), a fluorometric assay for double-stranded DNA (Qubit™), and agarose gel electrophoresis. The mean DNA yield per sample was found to be 114 μg from whole blood volumes that ranged from 0.5 to 4 mL. The mean A260/280 ratio and median A260/280 ratios were both 1.8. No correlation was found between the duration of storage and the total yield or the quality of DNA extracted. These data suggest that high-quality DNA can be extracted from whole blood samples that are stored at -30°C for up to 19 years.

Twitter Demographics

The data shown below were collected from the profiles of 11 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 11 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 11 100%

Demographic breakdown

Readers by professional status Count As %
Unspecified 3 27%
Student > Master 2 18%
Researcher 2 18%
Student > Postgraduate 2 18%
Other 1 9%
Other 1 9%
Readers by discipline Count As %
Unspecified 4 36%
Agricultural and Biological Sciences 4 36%
Biochemistry, Genetics and Molecular Biology 3 27%

Attention Score in Context

This research output has an Altmetric Attention Score of 6. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 10 January 2018.
All research outputs
#2,684,174
of 12,347,188 outputs
Outputs from Biopreservation and Biobanking
#48
of 205 outputs
Outputs of similar age
#90,423
of 354,973 outputs
Outputs of similar age from Biopreservation and Biobanking
#8
of 24 outputs
Altmetric has tracked 12,347,188 research outputs across all sources so far. Compared to these this one has done well and is in the 78th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 205 research outputs from this source. They typically receive more attention than average, with a mean Attention Score of 8.1. This one has done well, scoring higher than 75% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 354,973 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 74% of its contemporaries.
We're also able to compare this research output to 24 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 62% of its contemporaries.