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Systematic quantitative analysis of H2A and H2B variants by targeted proteomics

Overview of attention for article published in Epigenetics & Chromatin, January 2018
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4 tweeters

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30 Mendeley
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Title
Systematic quantitative analysis of H2A and H2B variants by targeted proteomics
Published in
Epigenetics & Chromatin, January 2018
DOI 10.1186/s13072-017-0172-y
Pubmed ID
Authors

Sara El Kennani, Annie Adrait, Olga Permiakova, Anne-Marie Hesse, Côme Ialy-Radio, Myriam Ferro, Virginie Brun, Julie Cocquet, Jérôme Govin, Delphine Pflieger

Abstract

Histones organize DNA into chromatin through a variety of processes. Among them, a vast diversity of histone variants can be incorporated into chromatin and finely modulate its organization and functionality. Classically, the study of histone variants has largely relied on antibody-based assays. However, antibodies have a limited efficiency to discriminate between highly similar histone variants. In this study, we established a mass spectrometry-based analysis to address this challenge. We developed a targeted proteomics method, using selected reaction monitoring or parallel reaction monitoring, to quantify a maximum number of histone variants in a single multiplexed assay, even when histones are present in a crude extract. This strategy was developed on H2A and H2B variants, using 55 peptides corresponding to 25 different histone sequences, among which a few differ by a single amino acid. The methodology was then applied to mouse testis extracts in which almost all histone variants are expressed. It confirmed the abundance profiles of several testis-specific histones during successive stages of spermatogenesis and the existence of predicted H2A.L.1 isoforms. This methodology was also used to explore the over-expression pattern of H2A.L.1 isoforms in a mouse model of male infertility. Our results demonstrate that targeted proteomics is a powerful method to quantify highly similar histone variants and isoforms. The developed method can be easily transposed to the study of human histone variants, whose abundance can be deregulated in various diseases.

Twitter Demographics

The data shown below were collected from the profiles of 4 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 30 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 30 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 8 27%
Student > Ph. D. Student 5 17%
Professor > Associate Professor 4 13%
Student > Doctoral Student 3 10%
Student > Bachelor 3 10%
Other 3 10%
Unknown 4 13%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 11 37%
Agricultural and Biological Sciences 6 20%
Chemistry 2 7%
Physics and Astronomy 1 3%
Medicine and Dentistry 1 3%
Other 1 3%
Unknown 8 27%

Attention Score in Context

This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 14 January 2018.
All research outputs
#7,136,516
of 12,362,966 outputs
Outputs from Epigenetics & Chromatin
#262
of 356 outputs
Outputs of similar age
#170,590
of 355,159 outputs
Outputs of similar age from Epigenetics & Chromatin
#20
of 28 outputs
Altmetric has tracked 12,362,966 research outputs across all sources so far. This one is in the 40th percentile – i.e., 40% of other outputs scored the same or lower than it.
So far Altmetric has tracked 356 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 6.6. This one is in the 23rd percentile – i.e., 23% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 355,159 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 48th percentile – i.e., 48% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 28 others from the same source and published within six weeks on either side of this one. This one is in the 25th percentile – i.e., 25% of its contemporaries scored the same or lower than it.