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Mendeley readers
| Chapter title |
Analysis of Histone Modifications in Acute Myeloid Leukaemia Using Chromatin Immunoprecipitation
|
|---|---|
| Chapter number | 15 |
| Book title |
Inflammation and Cancer
|
| Published in |
Methods in molecular biology, January 2018
|
| DOI | 10.1007/978-1-4939-7568-6_15 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-7567-9, 978-1-4939-7568-6
|
| Authors |
Benjamin J. Shields, Andrew Keniry, Marnie E. Blewitt, Matthew P. McCormack |
| Abstract |
Chromatin Immunoprecipitation (ChIP) using antibodies specific for histone modifications is a powerful technique for assessing the epigenetic states of cell populations by either quantitative PCR (ChIP-PCR) or next generation sequencing analysis (ChIP-Seq). Here we describe the procedure for ChIP of histone marks in myeloid leukaemia cell lines and the subsequent purification of genomic DNA associated with repressive and activating histone modifications for further analysis. This procedure can be widely applied to a variety of histone marks to assess both activating and repressive modifications in the context of myeloid leukaemia. |
Mendeley readers
The data shown below were compiled from readership statistics for 7 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 7 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Librarian | 1 | 14% |
| Lecturer | 1 | 14% |
| Student > Ph. D. Student | 1 | 14% |
| Researcher | 1 | 14% |
| Professor > Associate Professor | 1 | 14% |
| Other | 1 | 14% |
| Unknown | 1 | 14% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 2 | 29% |
| Agricultural and Biological Sciences | 2 | 29% |
| Neuroscience | 1 | 14% |
| Medicine and Dentistry | 1 | 14% |
| Unknown | 1 | 14% |