| Chapter title |
Assessing the cGAS-cGAMP-STING Activity of Cancer Cells
|
|---|---|
| Chapter number | 20 |
| Book title |
Inflammation and Cancer
|
| Published in |
Methods in molecular biology, January 2018
|
| DOI | 10.1007/978-1-4939-7568-6_20 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-7567-9, 978-1-4939-7568-6
|
| Authors |
Geneviève Pépin, Michael P. Gantier, Pépin, Geneviève, Gantier, Michael P. |
| Abstract |
DNA sensing by the STING pathway is emerging to be a crucial component of the antitumor immune response. Although it plays a key role in the activation of tumor immune cells, exactly how STING is activated by tumor cells is not fully understood. Recent evidence suggests that cGAS can be directly engaged and produces 2'3'-cyclic-GMP-AMP (cGAMP) within certain tumor cells upon stimulation with DNA damaging agents. Because cGAMP can transfer between adjacent cells, the capacity of tumor cells to produce cGAMP may activate tumor immune cells, even in the absence of functional STING signaling within the tumor. Here we describe a simple coculture protocol allowing for the functional characterization of cGAS/STING activity in tumor cells, together with cGAMP transfer to adjacent cells. This approach will help define how different tumors engage the STING pathway, and whether synthetic STING agonists should be used to potentiate the antitumor effects of chemotherapies. |
X Demographics
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Australia | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Scientists | 1 | 100% |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 27 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 10 | 37% |
| Researcher | 5 | 19% |
| Student > Bachelor | 4 | 15% |
| Student > Master | 2 | 7% |
| Lecturer | 1 | 4% |
| Other | 1 | 4% |
| Unknown | 4 | 15% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 10 | 37% |
| Agricultural and Biological Sciences | 6 | 22% |
| Immunology and Microbiology | 4 | 15% |
| Medicine and Dentistry | 2 | 7% |
| Pharmacology, Toxicology and Pharmaceutical Science | 1 | 4% |
| Other | 0 | 0% |
| Unknown | 4 | 15% |