| Chapter title |
Isolation and Characterization of Mouse Intrahepatic Lymphocytes by Flow Cytometry
|
|---|---|
| Chapter number | 23 |
| Book title |
Inflammation and Cancer
|
| Published in |
Methods in molecular biology, January 2018
|
| DOI | 10.1007/978-1-4939-7568-6_23 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-7567-9, 978-1-4939-7568-6
|
| Authors |
Florian Wiede, Tony Tiganis, Wiede, Florian, Tiganis, Tony |
| Abstract |
In the field of cellular immunology multicolor flow cytometry is a frequently applied method that allows for the simultaneously detection of multiple parameters on an individual cell basis. Flow cytometry can be used to characterize a wide range of immune cell subsets using fluorophore-conjugated antibodies to a wide range of cellular antigens. The isolation of immune cells from nonlymphoid tissue and their preparation for flow cytometry can be a challenging process with respect to immune cell yields and viability. Here we describe a method for the efficient isolation of viable mouse intrahepatic lymphocytes (IHL) from normal liver tissue and liver cancer and their subsequent characterization by multicolor flow cytometry. |
Mendeley readers
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|---|---|---|
| Unknown | 18 | 100% |
Demographic breakdown
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|---|---|---|
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| Student > Master | 3 | 17% |
| Other | 2 | 11% |
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| Other | 3 | 17% |
| Unknown | 3 | 17% |
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| Unknown | 5 | 28% |