Chapter title |
Expression and Purification of JAK1 and SOCS1 for Structural and Biochemical Studies
|
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Chapter number | 21 |
Book title |
Inflammation and Cancer
|
Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-7568-6_21 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7567-9, 978-1-4939-7568-6
|
Authors |
Nicholas P. D. Liau, Jeffrey J. Babon, Liau, Nicholas P. D., Babon, Jeffrey J. |
Abstract |
Interferon gamma (IFNγ) is a potent inflammatory and immune cytokine. IFNγ signals via the interferon gamma receptor (IFNGR), which is constitutively bound to Janus Kinase (JAK) 1 and JAK2 via its intracellular domain. These two JAK proteins then initiate the inflammatory signaling cascade. The most potent inhibitor of IFNγ signaling is Suppressor of Cytokine Signaling 1 (SOCS1). SOCS1 negatively regulates IFNγ signaling pathway (and other pathways) by directly inhibiting JAKs. Here, we describe a protocol for the recombinant production and purification of the JAK1 kinase domain and its inhibitor SOCS1, for structural and biochemical studies. |
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