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Employment of proteomic and immunological based methods for the identification of catalase as novel allergen from banana

Overview of attention for article published in Journal of Proteomics, March 2018
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  • Above-average Attention Score compared to outputs of the same age and source (52nd percentile)

Mentioned by

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2 tweeters

Citations

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8 Dimensions

Readers on

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28 Mendeley
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Title
Employment of proteomic and immunological based methods for the identification of catalase as novel allergen from banana
Published in
Journal of Proteomics, March 2018
DOI 10.1016/j.jprot.2018.01.007
Pubmed ID
Authors

Jasna Nikolić, Andrijana Nešić, Skadi Kull, Frauke Schocker, Uta Jappe, Marija Gavrović-Jankulović

Abstract

Diagnostic reagents based on food allergen extracts often lack sufficient sensitivity. The introduction of well characterized food allergens in molecular allergy diagnosis has been recognized as valid approach to circumvent unstandardized allergen extracts. Banana fruit (Musa acuminata) is a well-established allergen source which besides six characterized allergens, contains unidentified IgE reactive proteins whose clinical relevance remains undefined. By employment of a combinatorial peptide ligand library (CPLL) methodology with 2-D PAGE, mass spectrometric and 2-D immunoblot analysis, a novel allergen from banana fruit was detected in banana as catalase. A recombinant homologue of natural catalase was produced, isolated and biochemically characterized. The recombinant protein showed IgE reactivity in 7 out of 13 tested patients with suspected allergy to banana in immunoblot. Novel banana fruit allergens should be added as components to allergen-microarrays for the diagnosis and the monitoring of banana allergy. By employment of CPLL methodology with 2-D PAGE, mass spectrometric and 2-D immunoblot analysis catalase from banana fruit is identified as a novel allergen, with proposed designation as Mus a 7. IgE reactive recombinant Mus a 7 was produced and should be included in a component-resolved allergy diagnosis.

Twitter Demographics

The data shown below were collected from the profiles of 2 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 28 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 28 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 3 11%
Researcher 3 11%
Student > Master 3 11%
Other 2 7%
Student > Bachelor 2 7%
Other 2 7%
Unknown 13 46%
Readers by discipline Count As %
Agricultural and Biological Sciences 7 25%
Biochemistry, Genetics and Molecular Biology 4 14%
Immunology and Microbiology 1 4%
Social Sciences 1 4%
Medicine and Dentistry 1 4%
Other 1 4%
Unknown 13 46%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 15 January 2018.
All research outputs
#7,987,964
of 12,738,237 outputs
Outputs from Journal of Proteomics
#817
of 2,218 outputs
Outputs of similar age
#243,690
of 430,765 outputs
Outputs of similar age from Journal of Proteomics
#33
of 78 outputs
Altmetric has tracked 12,738,237 research outputs across all sources so far. This one is in the 23rd percentile – i.e., 23% of other outputs scored the same or lower than it.
So far Altmetric has tracked 2,218 research outputs from this source. They receive a mean Attention Score of 2.2. This one has gotten more attention than average, scoring higher than 54% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 430,765 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 33rd percentile – i.e., 33% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 78 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 52% of its contemporaries.