Title |
miR-27b inhibits fibroblast activation via targeting TGFβ signaling pathway
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Published in |
BMC Molecular and Cell Biology, January 2017
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DOI | 10.1186/s12860-016-0123-7 |
Pubmed ID | |
Authors |
Xiangming Zeng, Chaoqun Huang, Lakmini Senavirathna, Pengcheng Wang, Lin Liu |
Abstract |
MicroRNAs are a group of small RNAs that regulate gene expression at the posttranscriptional level. They regulate almost every aspect of cellular processes. In this study, we investigated whether miR-27b regulates pulmonary fibroblast activation. We found that miR-27b was down-regulated in fibrotic lungs and fibroblasts from an experimental mouse model of pulmonary fibrosis. The overexpression of miR-27b with a lentiviral vector inhibited TGFβ1-stimulated mRNA expression of collagens (COL1A1, COL3A1, and COL4A1) and alpha-smooth muscle actin, and protein expression of Col3A1 and alpha-smooth muscle actin in LL29 human pulmonary fibroblasts. miR-27b also reduced contractile activity of LL29. TGFβ receptor 1 and SMAD2 were identified as the targets of miR-27b by 3'-untranslated region luciferase reporter and western blotting assays. Our results suggest that miR-27b is an anti-fibrotic microRNA that inhibits fibroblast activation by targeting TGFβ receptor 1 and SMAD2. This discovery may provide new targets for therapeutic interventions of idiopathic pulmonary fibrosis. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 26 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 6 | 23% |
Student > Master | 5 | 19% |
Student > Doctoral Student | 4 | 15% |
Student > Bachelor | 2 | 8% |
Unknown | 9 | 35% |
Readers by discipline | Count | As % |
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Biochemistry, Genetics and Molecular Biology | 6 | 23% |
Agricultural and Biological Sciences | 4 | 15% |
Neuroscience | 3 | 12% |
Psychology | 1 | 4% |
Engineering | 1 | 4% |
Other | 0 | 0% |
Unknown | 11 | 42% |