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Construction and characterization of a recombinant yellow fever virus stably expressing Gaussia luciferase

Overview of attention for article published in Anais da Academia Brasileira de Ciências, July 2017
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Title
Construction and characterization of a recombinant yellow fever virus stably expressing Gaussia luciferase
Published in
Anais da Academia Brasileira de Ciências, July 2017
DOI 10.1590/0001-3765201720160196
Pubmed ID
Authors

TELISSA C. KASSAR, TEREZA MAGALHÃES, JOSÉ V.J. S. JÚNIOR, AMANDA G.O. CARVALHO, ANDRÉA N.M.R. DA SILVA, SABRINA R.A. QUEIROZ, GIOVANI R. BERTANI, LAURA H.V.G. GIL

Abstract

Yellow fever is an arthropod-borne viral disease that still poses high public health concerns, despite the availability of an effective vaccine. The development of recombinant viruses is of utmost importance for several types of studies, such as those aimed to dissect virus-host interactions and to search for novel antiviral strategies. Moreover, recombinant viruses expressing reporter genes may greatly facilitate these studies. Here, we report the construction of a recombinant yellow fever virus (YFV) expressing Gaussia luciferase (GLuc) (YFV-GLuc). We show, through RT-PCR, sequencing and measurement of GLuc activity, that stability of the heterologous gene was maintained after six passages. Furthermore, a direct association between GLuc expression and viral replication was observed (r2=0.9967), indicating that measurement of GLuc activity may be used to assess viral replication in different applications. In addition, we evaluated the use of the recombinant virus in an antiviral assay with recombinant human alfa-2b interferon. A 60% inhibition of GLuc expression was observed in cells infected with YFV-GLuc and incubated with IFN alfa-2b. Previously tested on YFV inhibition by plaque assays indicated a similar fold-decrease in viral replication. These results are valuable as they show the stability of YFV-GLuc and one of several possible applications of this construct.

Mendeley readers

The data shown below were compiled from readership statistics for 24 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 24 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 8 33%
Student > Ph. D. Student 5 21%
Student > Bachelor 3 13%
Other 2 8%
Student > Master 2 8%
Other 2 8%
Unknown 2 8%
Readers by discipline Count As %
Agricultural and Biological Sciences 5 21%
Biochemistry, Genetics and Molecular Biology 5 21%
Immunology and Microbiology 3 13%
Chemistry 2 8%
Computer Science 1 4%
Other 3 13%
Unknown 5 21%