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Cellular and molecular studies of the effects of a selective COX-2 inhibitor celecoxib in the cardiac cell line H9c2 and their correlation with death mechanisms.

Overview of attention for article published in Brazilian Journal of Medical and Biological Research, January 2014
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Title
Cellular and molecular studies of the effects of a selective COX-2 inhibitor celecoxib in the cardiac cell line H9c2 and their correlation with death mechanisms.
Published in
Brazilian Journal of Medical and Biological Research, January 2014
DOI 10.1590/1414-431x20133028
Pubmed ID
Authors

K.K. Sakane, C.J. Monteiro, W. Silva, A.R. Silva, P.M. Santos, K.F. Lima, K.C.M. Moraes

Abstract

Cardiovascular disease is one of the leading causes of death worldwide, and evidence indicates a correlation between the inflammatory process and cardiac dysfunction. Selective inhibitors of cyclooxygenase-2 (COX-2) enzyme are not recommended for long-term use because of potentially severe side effects to the heart. Considering this and the frequent prescribing of commercial celecoxib, the present study analyzed cellular and molecular effects of 1 and 10 µM celecoxib in a cell culture model. After a 24-h incubation, celecoxib reduced cell viability in a dose-dependent manner as also demonstrated in MTT assays. Furthermore, reverse transcription-polymerase chain reaction analysis showed that the drug modulated the expression level of genes related to death pathways, and Western blot analyses demonstrated a modulatory effect of the drug on COX-2 protein levels in cardiac cells. In addition, the results demonstrated a downregulation of prostaglandin E2 production by the cardiac cells incubated with celecoxib, in a dose-specific manner. These results are consistent with the decrease in cell viability and the presence of necrotic processes shown by Fourier transform infrared analysis, suggesting a direct correlation of prostanoids in cellular homeostasis and survival.

Mendeley readers

The data shown below were compiled from readership statistics for 13 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 13 100%

Demographic breakdown

Readers by professional status Count As %
Student > Postgraduate 2 15%
Student > Bachelor 2 15%
Student > Ph. D. Student 2 15%
Student > Master 1 8%
Researcher 1 8%
Other 2 15%
Unknown 3 23%
Readers by discipline Count As %
Medicine and Dentistry 3 23%
Nursing and Health Professions 1 8%
Biochemistry, Genetics and Molecular Biology 1 8%
Pharmacology, Toxicology and Pharmaceutical Science 1 8%
Agricultural and Biological Sciences 1 8%
Other 3 23%
Unknown 3 23%