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Characterization of Nanoparticles Intended for Drug Delivery

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Cover of 'Characterization of Nanoparticles Intended for Drug Delivery'

Table of Contents

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    Book Overview
  2. Altmetric Badge
    Chapter 1 Evaluating Nanomedicines: Obstacles and Advancements
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    Chapter 2 Detection of Bacterial Contamination in Nanoparticle Formulations by Agar Plate Test
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    Chapter 3 Considerations and Some Practical Solutions to Overcome Nanoparticle Interference with LAL Assays and to Avoid Endotoxin Contamination in Nanoformulations
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    Chapter 4 Elemental Analysis in Biological Matrices Using ICP-MS
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    Chapter 5 PEG Quantitation Using Reversed-Phase High-Performance Liquid Chromatography and Charged Aerosol Detection
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    Chapter 6 Quantitation of Surface Coating on Nanoparticles Using Thermogravimetric Analysis
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    Chapter 7 Immunoelectron Microscopy for Visualization of Nanoparticles
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    Chapter 8 Imaging of Liposomes by Transmission Electron Microscopy
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    Chapter 9 Updated Method for In Vitro Analysis of Nanoparticle Hemolytic Properties
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    Chapter 10 In Vitro Assessment of Nanoparticle Effects on Blood Coagulation
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    Chapter 11 In Vitro Analysis of Nanoparticle Effects on the Zymosan Uptake by Phagocytic Cells
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    Chapter 12 Assessing NLRP3 Inflammasome Activation by Nanoparticles
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    Chapter 13 Analysis of Complement Activation by Nanoparticles
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    Chapter 14 Methods for Analysis of Nanoparticle Immunosuppressive Properties In Vitro and In Vivo
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    Chapter 15 Analysis of Pro-inflammatory Cytokine and Type II Interferon Induction by Nanoparticles
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    Chapter 16 Analysis of Nanoparticle-Adjuvant Properties In Vivo
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    Chapter 17 In Vitro and In Vivo Methods for Analysis of Nanoparticle Potential to Induce Delayed-Type Hypersensitivity Reactions
  19. Altmetric Badge
    Chapter 18 Autophagy Monitoring Assay II: Imaging Autophagy Induction in LLC-PK1 Cells Using GFP-LC3 Protein Fusion Construct
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    Chapter 19 Improved Ultrafiltration Method to Measure Drug Release from Nanomedicines Utilizing a Stable Isotope Tracer
  21. Altmetric Badge
    Chapter 20 Designing an In Vivo Efficacy Study of Nanomedicines for Preclinical Tumor Growth Inhibition
Attention for Chapter 10: In Vitro Assessment of Nanoparticle Effects on Blood Coagulation
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Chapter title
In Vitro Assessment of Nanoparticle Effects on Blood Coagulation
Chapter number 10
Book title
Characterization of Nanoparticles Intended for Drug Delivery
Published in
Methods in molecular biology, January 2018
DOI 10.1007/978-1-4939-7352-1_10
Pubmed ID
Book ISBNs
978-1-4939-7350-7, 978-1-4939-7352-1
Authors

Timothy M. Potter, Jamie C. Rodriguez, Barry W. Neun, Anna N. Ilinskaya, Edward Cedrone, Marina A. Dobrovolskaia, Potter, Timothy M., Rodriguez, Jamie C., Neun, Barry W., Ilinskaya, Anna N., Cedrone, Edward, Dobrovolskaia, Marina A.

Abstract

Blood clotting is a complex process which involves both cellular and biochemical components. The key cellular players in the blood clotting process are thrombocytes or platelets. Other cells, including leukocytes and endothelial cells, contribute to clotting by expressing the so-called pro-coagulant activity (PCA) complex on their surface. The biochemical component of blood clotting is represented by the plasma coagulation cascade, which includes plasma proteins also known as coagulation factors. The coordinated interaction between platelets, leukocytes, endothelial cells, and plasma coagulation factors is necessary for maintaining hemostasis and for preventing excessive bleeding. Undesirable activation of all or some of these components may lead to pathological blood coagulation and life-threatening conditions such as consumptive coagulopathy or disseminated intravascular coagulation (DIC). In contrast, unintended inhibition of the coagulation pathways may lead to hemorrhage. Thrombogenicity is the property of a test material to induce blood coagulation by affecting one or more elements of the clotting process. Anticoagulant activity refers to the property of a test material to inhibit coagulation. The tendency to cause platelet aggregation, perturb plasma coagulation, and induce leukocyte PCA can serve as an in vitro measure of a nanomaterial's likelihood to be pro- or anticoagulant in vivo. This chapter describes three procedures for in vitro analyses of platelet aggregation, plasma coagulation time, and activation of leukocyte PCA. Platelet aggregation and plasma coagulation procedures have been described earlier. The revision here includes updated details about nanoparticle sample preparation, selection of nanoparticle concentration for the in vitro study, and updated details about assay controls. The chapter is expanded to describe a method for the leukocyte PCA analysis and case studies demonstrating the performance of these in vitro assays.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 26 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 26 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 5 19%
Professor 3 12%
Student > Master 2 8%
Professor > Associate Professor 2 8%
Researcher 2 8%
Other 2 8%
Unknown 10 38%
Readers by discipline Count As %
Chemistry 4 15%
Pharmacology, Toxicology and Pharmaceutical Science 3 12%
Biochemistry, Genetics and Molecular Biology 1 4%
Immunology and Microbiology 1 4%
Physics and Astronomy 1 4%
Other 2 8%
Unknown 14 54%