↓ Skip to main content

Circulating Tumor Cells

Overview of attention for book
Cover of 'Circulating Tumor Cells'

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter 1 Microfluidic Capture and Multiplex Immunofluorescence of Circulating Tumor Cells to Identify Cancer of Origin
  3. Altmetric Badge
    Chapter 2 Microfluidic Separation of Circulating Tumor Cells Based on Size and Deformability
  4. Altmetric Badge
    Chapter 3 A Novel Microfluidic Device for Isolation of Circulating Tumor Cells from Pancreatic Cancer Blood Samples
  5. Altmetric Badge
    Chapter 4 Microfluidic-Based Enrichment and Retrieval of Circulating Tumor Cells for RT-PCR Analysis
  6. Altmetric Badge
    Chapter 5 Microscale Laminar Vortices for High-Purity Extraction and Release of Circulating Tumor Cells
  7. Altmetric Badge
    Chapter 6 Separable Bilayer Microfiltration Device for Label-Free Enrichment of Viable Circulating Tumor Cells
  8. Altmetric Badge
    Chapter 7 Microfilter-Based Capture and Release of Viable Circulating Tumor Cells
  9. Altmetric Badge
    Chapter 8 Fourier Ptychographic Microscopy for Rapid, High-Resolution Imaging of Circulating Tumor Cells Enriched by Microfiltration
  10. Altmetric Badge
    Chapter 9 Automated Microfluidic Filtration and Immunocytochemistry Detection System for Capture and Enumeration of Circulating Tumor Cells and Other Rare Cell Populations in Blood
  11. Altmetric Badge
    Chapter 10 Filter-Adapted Fluorescent In Situ Hybridization (FA-FISH) for Filtration-Enriched Circulating Tumor Cells
  12. Altmetric Badge
    Chapter 11 Negative Enrichment and Isolation of Circulating Tumor Cells for Whole Genome Amplification
  13. Altmetric Badge
    Chapter 12 Capture and Genetic Analysis of Circulating Tumor Cells Using a Magnetic Separation Device (Magnetic Sifter)
  14. Altmetric Badge
    Chapter 13 RareCyte ® CTC Analysis Step 1: AccuCyte ® Sample Preparation for the Comprehensive Recovery of Nucleated Cells from Whole Blood
  15. Altmetric Badge
    Chapter 14 RareCyte ® CTC Analysis Step 2: Detection of Circulating Tumor Cells by CyteFinder ® Automated Scanning and Semiautomated Image Analysis
  16. Altmetric Badge
    Chapter 15 RareCyte® CTC Analysis Step 3: Using the CytePicker® Module for Individual Cell Retrieval and Subsequent Whole Genome Amplification of Circulating Tumor Cells for Genomic Analysis
  17. Altmetric Badge
    Chapter 16 Enumeration, Dielectrophoretic Capture, and Molecular Analysis of Circulating Tumor Cells
  18. Altmetric Badge
    Chapter 17 Enumeration of Circulating Tumor Cells and Disseminated Tumor Cells in Blood and Bone Marrow by Immunomagnetic Enrichment and Flow Cytometry (IE/FC)
  19. Altmetric Badge
    Chapter 18 Flow Cytometric Detection of Circulating Tumor Cells Using a Candidate Stem Cell Marker, p75 Neurotrophin Receptor (p75NTR)
  20. Altmetric Badge
    Chapter 19 Multispectral Imaging Analysis of Circulating Tumor Cells in Negatively Enriched Peripheral Blood Samples
  21. Altmetric Badge
    Chapter 20 Fiber-Optic Array Scanning Technology (FAST) for Detection and Molecular Characterization of Circulating Tumor Cells
  22. Altmetric Badge
    Chapter 21 A Noninvasive and Real-Time Method for Circulating Tumor Cell Detection by In Vivo Flow Cytometry
  23. Altmetric Badge
    Chapter 22 EpCAM-Independent Enrichment and Detection of Viable Circulating Tumor Cells Using the EPISPOT Assay
  24. Altmetric Badge
    Chapter 23 Utilizing Matrigel Transwell Invasion Assay to Detect and Enumerate Circulating Tumor Cells
  25. Altmetric Badge
    Chapter 24 Circulating Tumor Cells: Markers and Methodologies for Enrichment and Detection
Attention for Chapter 13: RareCyte ® CTC Analysis Step 1: AccuCyte ® Sample Preparation for the Comprehensive Recovery of Nucleated Cells from Whole Blood
Altmetric Badge

Citations

dimensions_citation
3 Dimensions

Readers on

mendeley
10 Mendeley
You are seeing a free-to-access but limited selection of the activity Altmetric has collected about this research output. Click here to find out more.
Chapter title
RareCyte ® CTC Analysis Step 1: AccuCyte ® Sample Preparation for the Comprehensive Recovery of Nucleated Cells from Whole Blood
Chapter number 13
Book title
Circulating Tumor Cells
Published in
Methods in molecular biology, January 2017
DOI 10.1007/978-1-4939-7144-2_13
Pubmed ID
Book ISBNs
978-1-4939-7143-5, 978-1-4939-7144-2
Authors

Arturo B. Ramirez, Lance U’Ren, Daniel E. Campton, David Stewart, Joshua J. Nordberg, Jackie L. Stilwell, Eric P. Kaldjian, Ramirez, Arturo B., U’Ren, Lance, Campton, Daniel E., Stewart, David, Nordberg, Joshua J., Stilwell, Jackie L., Kaldjian, Eric P.

Abstract

The RareCyte platform addresses important technology limitations of current circulating tumor cell (CTC) collection methods, and expands CTC interrogation to include advanced phenotypic characterization and single-cell molecular analysis. In this respect, it represents the "next generation" of cell-based liquid biopsy technologies. In order to identify and analyze CTCs, RareCyte has developed an integrated sample preparation, imaging and individual cell retrieval process. The first step in the process, AccuCyte(®), allows the separation, collection, and transfer to a slide the nucleated cell fraction of the blood that contains CTCs. Separation and collection are based on cell density-rather than size or surface molecular expression-and are performed within a closed system, without wash or lysis steps, enabling high CTC recovery. Here, we describe our technique for nucleated cell collection from a blood sample, and the spreading of these nucleated cells onto glass slides permitting immunofluorescent staining, cell identification, and individual cell picking described in subsequent chapters. In addition to collection of rare cells such as CTCs, AccuCyte also collects cells of the circulating immune system onto archivable slides as well as plasma from the same sample.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 10 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 10 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 3 30%
Student > Master 2 20%
Researcher 2 20%
Student > Doctoral Student 1 10%
Student > Postgraduate 1 10%
Other 0 0%
Unknown 1 10%
Readers by discipline Count As %
Medicine and Dentistry 4 40%
Biochemistry, Genetics and Molecular Biology 3 30%
Physics and Astronomy 1 10%
Pharmacology, Toxicology and Pharmaceutical Science 1 10%
Unknown 1 10%