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Candida albicans morphologies revealed by scanning electron microscopy analysis.

Overview of attention for article published in Brazilian Journal of Microbiology, December 2013
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Candida albicans morphologies revealed by scanning electron microscopy analysis.
Published in
Brazilian Journal of Microbiology, December 2013
DOI 10.1590/s1517-83822013005000056
Pubmed ID

Staniszewska M, Bondaryk M, Swoboda-Kopec E, Siennicka K, Sygitowicz G, Kurzatkowski W


Scanning electron microscope (SEM) observations were used to analyze particular morphologies of Candida albicans clinical isolate (strain 82) and mutants defective in hyphae-promoting genes EFG1 (strain HLC52) and/or CPH1 (strains HLC54 and Can16). Transcription factors Efg1 and Cph1 play role in regulating filamentation and adhesion of C. albicans' morphologies. Comparative analysis of such mutants and clinical isolate showed that Efg1 is required for human serum-induced cell growth and morphological switching. In the study, distinct differences between ultrastructural patterns of clinical strain's and null mutants' morphologies were observed (spherical vs tube-like blastoconidia, or solid and fragile constricted septa vs only the latter observed in strains with EFG1 deleted). In addition, wild type strain displayed smooth colonies of cells in comparison to mutants which exhibited wrinkled phenotype. It was observed that blastoconidia of clinical strain exhibited either polarly or randomly located budding. Contrariwise, morphotypes of mutants showed either multiple polar budding or a centrally located single bud scar (mother-daughter cell junction) distinguishing tube-like yeast/pseudohyphal growth (the length-to-width ratios larger than 1.5). In their planktonic form of growth, blastoconidia of clinical bloodstream isolate formed constitutively true hyphae under undiluted human serum inducing conditions. It was found that true hyphae are essential elements for developing structural integrity of conglomerate, as mutants displaying defects in their flocculation and conglomerate-forming abilities in serum. While filamentation is an important virulence trait in C. albicans the true hyphae are the morphologies which may be expected to play a role in bloodstream infections.

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Mendeley readers

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Geographical breakdown

Country Count As %
Serbia 1 1%
Ireland 1 1%
Unknown 80 98%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 19 23%
Student > Master 12 15%
Researcher 9 11%
Student > Ph. D. Student 9 11%
Other 6 7%
Other 15 18%
Unknown 12 15%
Readers by discipline Count As %
Medicine and Dentistry 22 27%
Biochemistry, Genetics and Molecular Biology 15 18%
Agricultural and Biological Sciences 12 15%
Pharmacology, Toxicology and Pharmaceutical Science 5 6%
Materials Science 3 4%
Other 7 9%
Unknown 18 22%