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Population Epigenetics

Overview of attention for book
Cover of 'Population Epigenetics'

Table of Contents

  1. Altmetric Badge
    Book Overview
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    Chapter 259 Nano-MeDIP-seq Methylome Analysis Using Low DNA Concentrations.
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    Chapter 260 Bisulfite Conversion of DNA from Tissues, Cell Lines, Buffy Coat, FFPE Tissues, Microdissected Cells, Swabs, Sputum, Aspirates, Lavages, Effusions, Plasma, Serum, and Urine
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    Chapter 261 Detecting Spatial Chromatin Organization by Chromosome Conformation Capture II: Genome-Wide Profiling by Hi-C
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    Chapter 262 Adjusting for Cell Type Composition in DNA Methylation Data Using a Regression-Based Approach
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    Chapter 263 Determining Epigenetic Targets: A Beginner's Guide to Identifying Genome Functionality Through Database Analysis.
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    Chapter 264 Analysis of Imprinted Gene Regulation.
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    Chapter 265 Library Construction for High-Throughput Mobile Element Identification and Genotyping.
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    Chapter 266 Correcting for Sample Heterogeneity in Methylome-Wide Association Studies.
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    Chapter 268 5-Hydroxymethylcytosine Profiling in Human DNA.
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    Chapter 269 Quantitative Analysis of Intra-chromosomal Contacts: The 3C-qPCR Method.
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    Chapter 285 The Design and Optimization of DNA Methylation Pyrosequencing Assays Targeting Region-Specific Repeat Elements.
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    Chapter 316 Statistical Methods for Methylation Data
Attention for Chapter 285: The Design and Optimization of DNA Methylation Pyrosequencing Assays Targeting Region-Specific Repeat Elements.
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Chapter title
The Design and Optimization of DNA Methylation Pyrosequencing Assays Targeting Region-Specific Repeat Elements.
Chapter number 285
Book title
Population Epigenetics
Published in
Methods in molecular biology, August 2015
DOI 10.1007/7651_2015_285
Pubmed ID
26246355
Book ISBNs
978-1-4939-6901-2, 978-1-4939-6903-6
Authors

Hoad, Gwen, Harrison, Kristina, Gwen Hoad, Kristina Harrison

Abstract

Epigenetic modifications, such as DNA methylation, can contribute to gene regulation and chromosomal stability. There are several methods and techniques available for methylation analysis, ranging from global methylation to gene-specific targeted regions. Bisulfite conversion enables numerous methodologies to be used for downstream applications, including pyrosequencing which measures DNA methylation at an individual CpG site level. This allows specific regions of interest to be targeted for DNA methylation analysis. Designing and optimizing pyrosequencing assays correctly is vital for the interpretation of results.Dysregulation of DNA methylation has been implicated in human diseases, with regions such as repeat elements commonly altered. Human population studies investigating these tend to use consensus sequences to target repeat elements. However, these elements have high mutational rates, particularly Alu sequences, which could lead to assay bias and masking of changes at a regional level. Therefore, it may be more beneficial to target specific repeat elements depending upon their chromosomal location, rather than analyzing overall methylation levels.

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X Demographics

The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
 Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 8 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 8 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 2 25%
Professor 1 13%
Professor > Associate Professor 1 13%
Student > Master 1 13%
Unknown 3 38%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 2 25%
Nursing and Health Professions 1 13%
Agricultural and Biological Sciences 1 13%
Medicine and Dentistry 1 13%
Unknown 3 38%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 08 August 2015.
All research outputs
#20,286,650
of 22,821,814 outputs
Outputs from Methods in molecular biology
#9,915
of 13,124 outputs
Outputs of similar age
#220,873
of 264,036 outputs
Outputs of similar age from Methods in molecular biology
#16
of 18 outputs
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So far Altmetric has tracked 13,124 research outputs from this source. They receive a mean Attention Score of 3.4. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
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We're also able to compare this research output to 18 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.

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