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Heterologous and endogenous U6 snRNA promoters enable CRISPR/Cas9 mediated genome editing in Aspergillus niger

Overview of attention for article published in Fungal Biology and Biotechnology, February 2018
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  • In the top 25% of all research outputs scored by Altmetric
  • Among the highest-scoring outputs from this source (#29 of 157)
  • High Attention Score compared to outputs of the same age (83rd percentile)
  • High Attention Score compared to outputs of the same age and source (99th percentile)

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Title
Heterologous and endogenous U6 snRNA promoters enable CRISPR/Cas9 mediated genome editing in Aspergillus niger
Published in
Fungal Biology and Biotechnology, February 2018
DOI 10.1186/s40694-018-0047-4
Pubmed ID
Authors

Xiaomei Zheng, Ping Zheng, Jibin Sun, Zhang Kun, Yanhe Ma

Abstract

U6 promoters have been used for single guide RNA (sgRNA) transcription in the clustered regularly interspaced short palindromic repeats/CRISPR-associated protein (CRISPR/Cas9) genome editing system. However, no availableU6promoters have been identified inAspergillus niger,which is an important industrial platform for organic acid and protein production. Two CRISPR/Cas9 systems established inA. nigerhave recourse to the RNA polymerase II promoter or in vitro transcription for sgRNA synthesis, but these approaches generally increase cloning efforts and genetic manipulation. The validation of functional RNA polymerase II promoters is therefore an urgent need forA. niger. Here, we developed a novel CRISPR/Cas9 system inA. nigerfor sgRNA expression, based on one endogenousU6promoter and two heterologousU6promoters. The three testedU6promoters enabled sgRNA transcription and the disruption of the polyketide synthasealbAgene inA. niger. Furthermore, this system enabled highly efficient gene insertion at the targeted genome loci inA. nigerusing donor DNAs with homologous arms as short as 40-bp. This study demonstrated that both heterologous and endogenousU6promoters were functional for sgRNA expression inA. niger. Based on this result, a novel and simple CRISPR/Cas9 toolbox was established inA. niger,that will benefit future gene functional analysis and genome editing.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 66 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 66 100%

Demographic breakdown

Readers by professional status Count As %
Student > Master 12 18%
Researcher 10 15%
Student > Ph. D. Student 10 15%
Student > Bachelor 7 11%
Student > Doctoral Student 2 3%
Other 4 6%
Unknown 21 32%
Readers by discipline Count As %
Agricultural and Biological Sciences 16 24%
Biochemistry, Genetics and Molecular Biology 16 24%
Chemical Engineering 2 3%
Engineering 2 3%
Medicine and Dentistry 2 3%
Other 4 6%
Unknown 24 36%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 11. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 27 July 2023.
All research outputs
#3,149,190
of 24,293,076 outputs
Outputs from Fungal Biology and Biotechnology
#29
of 157 outputs
Outputs of similar age
#71,726
of 447,056 outputs
Outputs of similar age from Fungal Biology and Biotechnology
#1
of 5 outputs
Altmetric has tracked 24,293,076 research outputs across all sources so far. Compared to these this one has done well and is in the 86th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 157 research outputs from this source. They typically receive more attention than average, with a mean Attention Score of 7.6. This one has done well, scoring higher than 82% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 447,056 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 83% of its contemporaries.
We're also able to compare this research output to 5 others from the same source and published within six weeks on either side of this one. This one has scored higher than all of them