Title |
Isolation of xylose isomerases by sequence- and function-based screening from a soil metagenomic library
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Published in |
Biotechnology for Biofuels and Bioproducts, May 2011
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DOI | 10.1186/1754-6834-4-9 |
Pubmed ID | |
Authors |
Nádia Skorupa Parachin, Marie F Gorwa-Grauslund |
Abstract |
Xylose isomerase (XI) catalyses the isomerisation of xylose to xylulose in bacteria and some fungi. Currently, only a limited number of XI genes have been functionally expressed in Saccharomyces cerevisiae, the microorganism of choice for lignocellulosic ethanol production. The objective of the present study was to search for novel XI genes in the vastly diverse microbial habitat present in soil. As the exploitation of microbial diversity is impaired by the ability to cultivate soil microorganisms under standard laboratory conditions, a metagenomic approach, consisting of total DNA extraction from a given environment followed by cloning of DNA into suitable vectors, was undertaken. |
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Other | 0 | 0% |
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Demographic breakdown
Readers by professional status | Count | As % |
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Researcher | 34 | 22% |
Student > Ph. D. Student | 32 | 21% |
Student > Master | 23 | 15% |
Student > Bachelor | 19 | 12% |
Student > Doctoral Student | 10 | 6% |
Other | 21 | 14% |
Unknown | 16 | 10% |
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Immunology and Microbiology | 3 | 2% |
Other | 11 | 7% |
Unknown | 19 | 12% |